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Sample GSM675130 Query DataSets for GSM675130
Status Public on Dec 31, 2011
Title 1C5HX
Sample type RNA
Source name U87 glioma cells
Organism Homo sapiens
Characteristics cell line: U87-MG
plasmid: pCDNA3.1_IRE1DN
treatment: under hypoxia
Treatment protocol cells were washed with ice cold PBS
Growth protocol U87 control and IRE1DN cells subjected to severe hypoxia (0.1%), glucose deprivation, glutamine deprivation and tunicamycin treatment (5ug/ml) for 16h
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions. RNA was purified on Quiagen columns
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Human U133A 2.0 Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol standard Affy protocol
Description Gene expression data from U87_IRE1DN cells under hypoxia
Data processing GCOS 1.2 software (Affymetrix).
Submission date Feb 14, 2011
Last update date Dec 31, 2011
Contact name eric chevet
Organization name inserm
Department U1053
Street address 146 rue Leo Saignat
City Bordeaux
ZIP/Postal code 33076
Country France
Platform ID GPL571
Series (1)
GSE27306 IRE1-dependent transcriptome remodelling upon ER stress in human glioma cells

Data table header descriptions
VALUE signal intensity

Data table
AFFX-BioB-5_at 62.4
AFFX-BioB-M_at 74.1
AFFX-BioB-3_at 48.2
AFFX-BioC-5_at 169
AFFX-BioC-3_at 226.6
AFFX-BioDn-5_at 338.1
AFFX-BioDn-3_at 708
AFFX-CreX-5_at 1846.1
AFFX-CreX-3_at 2114.8
AFFX-DapX-5_at 95.3
AFFX-DapX-M_at 171.8
AFFX-DapX-3_at 168.5
AFFX-LysX-5_at 10.7
AFFX-LysX-M_at 15.6
AFFX-LysX-3_at 34.2
AFFX-PheX-5_at 20.4
AFFX-PheX-M_at 23.7
AFFX-PheX-3_at 17.4
AFFX-ThrX-5_at 22.7
AFFX-ThrX-M_at 34.8

Total number of rows: 22277

Table truncated, full table size 339 Kbytes.

Supplementary file Size Download File type/resource
GSM675130.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table
Processed data are available on Series record

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