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Sample GSM674308 Query DataSets for GSM674308
Status Public on Apr 01, 2011
Title non-smoker 343PL
Sample type RNA
Source name term placenta, non-smoker
Organism Homo sapiens
Characteristics tissue: term placenta
smoking status: non-smoker
age (years): 35
maternal bmi: 21
parity: 4
gestational age (weeks): 41
mode of delivery: vaginal
placental weight (g): 500
newborn weight (g): 3880
apgar score (5s): 10
maternal blood cotinine (ng/ml): 0.28
cord blood cotinine (ng/ml): 0.18
individual: 343
Extracted molecule total RNA
Extraction protocol The middle parts of placenta sections were frozen in RNA Later solution (Ambion) and stored at –20°C until RNA isolation. Placental total RNA was isolated using the RNeasy Mini Kit (Qiagen, Hilden, Germany) according to the manufacturer instructions. Integrity of the total RNAs was assayed by the Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA), and only samples with RNA integrity number (RIN) above 7.0 were used for gene expression profiling.
Label biotin
Label protocol Biotinylated cRNA was prepared from 200 ng of total RNA using the Illumina TotalPrep RNA Amplification Kit (Ambion). In vitro transcription (IVT) was performed at 37°C for 14 hours.
Hybridization protocol 750 ng of biotinylated cRNA was hybridized to the array for 17 hours according to the manufacturer manual. Final staining was performed with streptavidin conjugated to Cy-3 fluorescent dye.
Scan protocol Arrays were scanned using BeadArray Reader (Illumina), and bead level data were extracted by BeadStudio Software (Illumina).
Description 343PL
Middle part of the placenta villi parenchyma sections; 5-cm away from the site of cord insertion.
Data processing Bead summary data were imported into the R statistical environment ( and normalized by the quantile method in the Lumi package. Only probes that reached detection P-value < 0.01 in all samples were used for further analyses. Differentially expressed genes were analysed in the Limma package. A linear model was fitted for each gene given a series of arrays using the lmFit function. Using the Benjamini and Hochberg method, P-values were corrected for multiple testing. The M, PL, and D groups were normalized separately.
Submission date Feb 13, 2011
Last update date Apr 01, 2011
Contact name Hana Bruchova
Phone +420221977306
Fax +420221977371
Organization name Institute of Hematology and Transfusion
Department Molecular Genetics
Lab Genomics
Street address U nemocnice 1
City Prague 2
ZIP/Postal code 128 20
Country Czech Republic
Platform ID GPL6883
Series (1)
GSE27272 Comprehensive Study of Tobacco Smoke-Related Transcriptome Alterations in Maternal and Fetal Cells

Data table header descriptions
VALUE Normalized data

Data table
ILMN_1802380 7.279256161
ILMN_1792389 1.602397231
ILMN_2375156 2.749369444
ILMN_1697642 8.481618461
ILMN_1681845 7.986513094
ILMN_1690979 4.233889238
ILMN_1811114 3.101462374
ILMN_1660729 8.783958771
ILMN_2129572 3.768516764
ILMN_1705659 2.065719634
ILMN_1674774 0.939052186
ILMN_1702329 1.165643642
ILMN_1658806 1.509733731
ILMN_2310896 0.860536173
ILMN_1675927 6.6759718
ILMN_2109994 8.193967627
ILMN_1745256 9.594400808
ILMN_2191313 4.09213321
ILMN_1689123 8.651566464
ILMN_1674337 9.877247768

Total number of rows: 24526

Table truncated, full table size 596 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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