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Sample GSM674222 Query DataSets for GSM674222
Status Public on Apr 01, 2011
Title smoker 356M
Sample type RNA
Source name maternal peripheral blood, smoker
Organism Homo sapiens
Characteristics tissue: maternal peripheral blood
smoking status: smoker
age (years): 21.678302532512
maternal bmi: 19.379197433927
parity: 1
gestational age (weeks): 40
mode of delivery: vaginal
placental weight (g): 520
newborn weight (g): 3200
apgar score (5s): 10
maternal blood cotinine (ng/ml): 68.4435581402562
cord blood cotinine (ng/ml): 80.1063840939639
individual: 356
Extracted molecule total RNA
Extraction protocol Peripheral blood was sampled and processed using the LeukoLOCK™ Total RNA Isolation System (Ambion, Austin, TX, USA) according to the manufacturer manual. The system employs filter-based leukocyte-depletion technology to isolate leukocytes from whole blood and RNAlater™ (Ambion) to stabilize the cells on the filter. By removal of red blood cells, the RNA purified from captured leukocytes is inherently depleted of globin mRNA, which improves sample quality for expression profiling and other applications. Integrity of the total RNAs was assayed by the Bioanalyzer 2100 (Agilent Technologies, Santa Clara, CA, USA), and only samples with RNA integrity number (RIN) above 7.0 were used for gene expression profiling.
Label biotin
Label protocol Biotinylated cRNA was prepared from 200 ng of total RNA using the Illumina TotalPrep RNA Amplification Kit (Ambion). In vitro transcription (IVT) was performed at 37°C for 14 hours.
Hybridization protocol 750 ng of biotinylated cRNA was hybridized to the array for 17 hours according to the manufacturer manual. Final staining was performed with streptavidin conjugated to Cy-3 fluorescent dye.
Scan protocol Arrays were scanned using BeadArray Reader (Illumina), and bead level data were extracted by BeadStudio Software (Illumina).
Description 356M
Data processing Bead summary data were imported into the R statistical environment ( and normalized by the quantile method in the Lumi package. Only probes that reached detection P-value < 0.01 in all samples were used for further analyses. Differentially expressed genes were analysed in the Limma package. A linear model was fitted for each gene given a series of arrays using the lmFit function. Using the Benjamini and Hochberg method, P-values were corrected for multiple testing. The M, PL, and D groups were normalized separately.
Submission date Feb 13, 2011
Last update date Apr 01, 2011
Contact name Hana Bruchova
Phone +420221977306
Fax +420221977371
Organization name Institute of Hematology and Transfusion
Department Molecular Genetics
Lab Genomics
Street address U nemocnice 1
City Prague 2
ZIP/Postal code 128 20
Country Czech Republic
Platform ID GPL6883
Series (1)
GSE27272 Comprehensive Study of Tobacco Smoke-Related Transcriptome Alterations in Maternal and Fetal Cells

Data table header descriptions
VALUE Normalized data

Data table
ILMN_1802380 9.495037459
ILMN_1792389 6.247630156
ILMN_2375156 3.975015053
ILMN_1697642 5.183534095
ILMN_1681845 8.244275841
ILMN_1690979 3.194902418
ILMN_1811114 2.404272113
ILMN_1660729 1.178483083
ILMN_2129572 1.583530149
ILMN_1705659 1.923108557
ILMN_1674774 1.596578011
ILMN_1702329 1.37787774
ILMN_1658806 2.404272113
ILMN_2310896 8.134590152
ILMN_1675927 1.51763384
ILMN_2109994 7.410656589
ILMN_1745256 8.488334429
ILMN_2191313 3.467360861
ILMN_1689123 9.091891532
ILMN_1674337 6.980036839

Total number of rows: 24526

Table truncated, full table size 595 Kbytes.

Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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