|
Status |
Public on Jan 15, 2023 |
Title |
TCR-CD8_WT_Liv |
Sample type |
SRA |
|
|
Source name |
Liver
|
Organism |
Mus musculus |
Characteristics |
cell type: CD8 T cell tissue: Liver genotype: WT strain background: C57BL/6 library type: TCR
|
Extracted molecule |
total RNA |
Extraction protocol |
Single-cell suspension from splenocytes and liver T cells from three C57BL/6 mice and three EomesTg/Tg mice were obtained as described. Cells from each strain were pooled and CD8+ T cells from spleens and livers were sorted. Microscope assessment after Trypan blue staining allowed to count viable cells following FACS sorting. Cell preparations were centrifuged at 500 RCF for 7 min and pellets were resuspended in 10%FCS/RPMI medium. Cells were loaded on the Chromium Controller (10x Genomics) Single-cell RNA-seq libraries were prepared using the Chromium Single Cell 5′ v1 Reagent Kit (10x Genomics) according to manufacturer’s protocol.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic single cell |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
|
|
Description |
cr-mm-MLE3-TCR-CD8_WT_Liv-D1 10x Genomics
|
Data processing |
Cell Ranger (10x Genomics) functions mkfastq and count were used to align to the mm10 genome and demultiplex the sequencing data and generate gene-barcode matrices. Assembly: mm10 Supplementary files format and content: Cell ranger output files
|
|
|
Submission date |
Nov 14, 2022 |
Last update date |
Jan 15, 2023 |
Contact name |
Abdulkader AZOUZ |
E-mail(s) |
Abdulkader.azouz@ulb.be
|
Organization name |
Université Libre de Bruxelles
|
Street address |
Rue Adrienne Bolland, 8
|
City |
GOSSELIES |
ZIP/Postal code |
6041 |
Country |
Belgium |
|
|
Platform ID |
GPL24247 |
Series (2) |
GSE217985 |
Homeostatic PD-1 signalling restrains Eomes-dependent oligoclonal expansion of liver- resident CD8 T cells [scRNA-seq] |
GSE217986 |
Homeostatic PD-1 signalling restrains Eomes-dependent oligoclonal expansion of liver- resident CD8 T cells. |
|
Relations |
BioSample |
SAMN31719882 |
SRA |
SRX18264836 |