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Sample GSM6732152 Query DataSets for GSM6732152
Status Public on Jan 15, 2023
Title TCR-CD8_WT_Liv
Sample type SRA
 
Source name Liver
Organism Mus musculus
Characteristics cell type: CD8 T cell
tissue: Liver
genotype: WT
strain background: C57BL/6
library type: TCR
Extracted molecule total RNA
Extraction protocol Single-cell suspension from splenocytes and liver T cells from three C57BL/6 mice and three EomesTg/Tg mice were obtained as described. Cells from each strain were pooled and CD8+ T cells from spleens and livers were sorted. Microscope assessment after Trypan blue staining allowed to count viable cells following FACS sorting. Cell preparations were centrifuged at 500 RCF for 7 min and pellets were resuspended in 10%FCS/RPMI medium. Cells were loaded on the Chromium Controller (10x Genomics)
Single-cell RNA-seq libraries were prepared using the Chromium Single Cell 5′ v1 Reagent Kit (10x Genomics) according to manufacturer’s protocol.
 
Library strategy RNA-Seq
Library source transcriptomic single cell
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Description cr-mm-MLE3-TCR-CD8_WT_Liv-D1
10x Genomics
Data processing Cell Ranger (10x Genomics) functions mkfastq and count were used to align to the mm10 genome and demultiplex the sequencing data and generate gene-barcode matrices.
Assembly: mm10
Supplementary files format and content: Cell ranger output files
 
Submission date Nov 14, 2022
Last update date Jan 15, 2023
Contact name Abdulkader AZOUZ
E-mail(s) Abdulkader.azouz@ulb.be
Organization name Université Libre de Bruxelles
Street address Rue Adrienne Bolland, 8
City GOSSELIES
ZIP/Postal code 6041
Country Belgium
 
Platform ID GPL24247
Series (2)
GSE217985 Homeostatic PD-1 signalling restrains Eomes-dependent oligoclonal expansion of liver- resident CD8 T cells [scRNA-seq]
GSE217986 Homeostatic PD-1 signalling restrains Eomes-dependent oligoclonal expansion of liver- resident CD8 T cells.
Relations
BioSample SAMN31719882
SRA SRX18264836

Supplementary file Size Download File type/resource
GSM6732152_filtered_contig_annotations_WT_Liv.csv.gz 263.4 Kb (ftp)(http) CSV
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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