|
| Status |
Public on Jan 15, 2023 |
| Title |
GEX-CD8_EomesTG_Sp |
| Sample type |
SRA |
| |
|
| Source name |
Spleen
|
| Organism |
Mus musculus |
| Characteristics |
cell type: CD8 T cell
tissue: Spleen
genotype: Eomes TG
strain background: C57BL/6
library type: GEX
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Single-cell suspension from splenocytes and liver T cells from three C57BL/6 mice and three EomesTg/Tg mice were obtained as described. Cells from each strain were pooled and CD8+ T cells from spleens and livers were sorted. Microscope assessment after Trypan blue staining allowed to count viable cells following FACS sorting. Cell preparations were centrifuged at 500 RCF for 7 min and pellets were resuspended in 10%FCS/RPMI medium. Cells were loaded on the Chromium Controller (10x Genomics)
Single-cell RNA-seq libraries were prepared using the Chromium Single Cell 5′ v1 Reagent Kit (10x Genomics) according to manufacturer’s protocol.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
cr-mm-MLE2-GEX-CD8_EomesTG_Sp-C2
10x Genomics
|
| Data processing |
Cell Ranger (10x Genomics) functions mkfastq and count were used to align to the mm10 genome and demultiplex the sequencing data and generate gene-barcode matrices.
Assembly: mm10
Supplementary files format and content: Cell ranger output files
|
| |
|
| Submission date |
Nov 14, 2022 |
| Last update date |
Jan 15, 2023 |
| Contact name |
Abdulkader AZOUZ |
| E-mail(s) |
Abdulkader.azouz@ulb.be
|
| Organization name |
Université Libre de Bruxelles
|
| Street address |
Rue Adrienne Bolland, 8
|
| City |
GOSSELIES |
| ZIP/Postal code |
6041 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL24247 |
| Series (2) |
| GSE217985 |
Homeostatic PD-1 signalling restrains Eomes-dependent oligoclonal expansion of liver- resident CD8 T cells [scRNA-seq] |
| GSE217986 |
Homeostatic PD-1 signalling restrains Eomes-dependent oligoclonal expansion of liver- resident CD8 T cells. |
|
| Relations |
| BioSample |
SAMN31719885 |
| SRA |
SRX18264833 |
