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Sample GSM6726731 Query DataSets for GSM6726731
Status Public on Nov 15, 2023
Title WholeRoot_625µMP_1d_rep2 [sP1d.2]
Sample type RNA
 
Source name Whole root, 625 µM P, 1 days, replicate 2
Organism Arabidopsis thaliana
Characteristics tissue: whole root
treatment: 625 µM P
time after treatment: 1 day
Treatment protocol Seven-day-old seedlings precultured on sufficient phosphorus were transferred to fresh solid half-strength Murashige and Skoog medium containing 625 µM KH2PO4 (sufficient P) or 100 µM KH2PO4 (deficient P). The missing potassium in the P-deficient medium was supplied as KCl.
Growth protocol Seeds of Arabidopsis thaliana (Columbia-0, CS60000) were surface sterilized in 70% (v/v) ethanol and 0.05% (v/v) Triton X-100. Seedlings were first germinated for seven days on agar plates containing 0.5% (w/v) sucrose and 1% (w/v) agar before seedlings of similar sizes were transferred to treatments containing identical sucrose and agar concentrations. The nutrient composition was half-strenght Murashige and Skoog and Difco agar (Becton Dickinson; cat. number 214541;) was used as gelling agent. In the preculture, 625 µM P was supplied as KH2PO4. In treatment plates, the solid half-strength MS medium contained 625 µM P (sufficient P) or 100 µM P (deficient P), with 525 µM K being supplied as KCl. Each treatment plate contained 10 seven-day-old seedling. Each replicate was composed of 5 plates containing 10 seven-day-old seedling each (i.e., 50 plants pooled as one replicate). Agar plates were sealed with Leukopor tape (Smith & Nephew) and placed vertically in a randomized block design within a growth cabinet (Percival Scientific) with 22°C day and 19°C night temperatures and a 10-h light period with a light intensity of 120 µmol photons m-2 s-1.
Extracted molecule total RNA
Extraction protocol RNA was extracted from whole roots (roots of 50 plants pooled togeher to form one replicate) using an RNeasy plant mini kit (Qiagen) with on-column DNase treatment according to the manufacturer’s instructions. cDNA was prepared using random hexamer primers and SuperScript II reverse transcriptase (Life Technologies) according to the manufacturer’s protocol.
Label Cy3
Label protocol RNA amplification, labeling and hybridization to Agilent microarrays (Arabidopsis (V4, 021169) Gene Expression Microarray) were conducted following the manufacturer’s protocol (Agilent Technologies).
 
Hybridization protocol RNA amplification, labeling and hybridization to Agilent microarrays (Arabidopsis (V4, 021169) Gene Expression Microarray) were conducted following the manufacturer’s protocol (Agilent Technologies).
Scan protocol Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner using one color scan setting for 1x44k array slides.
Description Gene expression after 1 day of 625 µM posphate treatment
Data processing The scanned images were analyzed with Feature Extraction Software 11.5.1.1 (Agilent) using default parameters (protocol GE1_1105_Oct12 and Grid: 021169_D_F_20140929) to obtain background subtracted and spatially detrended Processed Signal intensities. Analysis of microarray data was performed with the R package limma. Raw feature intensities were background corrected using “normexp”, and “quantile” normalization method was used to normalize between arrays.
 
Submission date Nov 11, 2022
Last update date Nov 15, 2023
Contact name Anja Hartmann
E-mail(s) hartmann@ipk-gatersleben.de
Phone +49 39482 5-578
Organization name IPK Gatersleben
Department PZB
Lab MPE
Street address Corrensstraße 3
City Seeland
State/province Saxony-Anhalt
ZIP/Postal code 06466
Country Germany
 
Platform ID GPL12621
Series (1)
GSE217790 Expression signatures of Arabidopsis thaliana roots under low or high phosphate conditions.

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
A_84_P10000 2.975906385
A_84_P10001 5.051629491
A_84_P10002 8.271470333
A_84_P10004 3.228365512
A_84_P10005 10.33682854
A_84_P100056 10.99265224
A_84_P10006 4.190680331
A_84_P10007 10.77819252
A_84_P10008 9.595407186
A_84_P10009 3.676529338
A_84_P10010 7.563331808
A_84_P10011 4.877730161
A_84_P10012 2.975906385
A_84_P10013 5.521431476
A_84_P10014 2.674420247
A_84_P10015 14.3265034
A_84_P100156 4.041290158
A_84_P10016 7.235386592
A_84_P10017 9.419592905
A_84_P10018 3.228365512

Total number of rows: 35334

Table truncated, full table size 846 Kbytes.




Supplementary file Size Download File type/resource
GSM6726731_US91603673_252116914283_S01_GE1_1105_Oct12_1_4.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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