|Public on Nov 28, 2023
|Mouse immature neurons
|cell type: immature neurons
chip antibody: None
|These primary immature neurons were either untreated (Control), or treated with 10mM 2-DG for 6h.
|Primary immature neurons were cultured from E14 mouse embryos and plated on poly-D-Lysine coated plates in the EMEM plus GlutMAX medium added with 10% fetalk bovine serum, 5% horse serum, and 1% Penicillin/Streptomycin. These neurons (two days in vitro) were treated with 2-Deoxyglucose (2-DG).
|Purified RNA was extracted from these samples using Nucleospin RNA kit (Macherey-Nagel) and follwing its protocol. RNA concentration determined by Nanodrop 1000 (Thermofisher scientific).
Libraries were prepared according to Illumina's instructions
|Illumina NovaSeq 6000
|BWA was used for alignment
Picard was used for removing duplicates
MACS2 was used for peak calling
DiffBind for differential peak calling
Supplementary files format and content: peaks
|Nov 04, 2022
|Last update date
|Nov 28, 2023
|University of California, Los Angeles
|Department of Psychiatry and Neurology Department of Psychiatry
|AMRF Functional Genomics Common Research Resource
|760 Westwood Plaza, Room 37-420
|The Intermittent Fasting Mimetic, 2-deoxyglucose, Reprograms Brain Derived Neurotrophic Factor (BDNF) transcription via an Adaptive ER Stress-ATF4 Pathway and Improves Outcomes in Alzheimer’s and Stroke Models [ChIP-seq]
|The Intermittent Fasting Mimetic, 2-deoxyglucose, Reprograms Brain Derived Neurotrophic Factor (BDNF) transcription via an Adaptive ER Stress-ATF4 Pathway and Improves Outcomes in Alzheimer’s and Stroke Models