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Sample GSM669967 Query DataSets for GSM669967
Status Public on Mar 31, 2011
Title ChIP-Seq analysis of H3K4me3 in HUES64 cells; Lib:MC:20101025:02--ChIP:MC:20101020:02:ES cell HUES_64:H3K4Me3
Sample type SRA
 
Source name Harvard University Embryonic Stem Cells; Lib:MC:20101025:02--ChIP:MC:20101020:02:ES cell HUES_64:H3K4Me3
Organism Homo sapiens
Characteristics sample alias: 196
sample common name: HUES64 Cell Line
lineage: embrionic stem cell
medium: KSR
molecule: genomic DNA
disease: none
passage: NA
line: HUES64
differentiation_method: NA
batch: ES cell HUES_64-1
biomaterial_type: Cell Line
differentiation_stage: undifferentiated
Sex: Unknown
biomaterial_provider: Harvard University
chip_protocol_bead_type: Protein A-Sepharose 4B
chip_antibody: H3K4Me3
chip_protocol_chromatin_amount: 10 million cells
extraction_protocol: SDS lysis
chip_protocol_antibody_amount: 2-15 ug
chip_protocol_bead_amount: 70 ul bed volume
chip_protocol: Bernstein_BROAD_ENCODE_protocol
extraction_protocol_sonication_cycles: 1
chip_antibody_catalog: 07-473
chip_antibody_provider: Millipore
chip_antibody_lot: DAM1623866
experiment_type: Histone H3K4me3
extraction_protocol_type_of_sonicator: Bioruptor
Extracted molecule genomic DNA
Extraction protocol Library construction protocol: Single read - Illumina
 
Library strategy ChIP-Seq
Library source genomic
Library selection ChIP
Instrument model Illumina Genome Analyzer IIx
 
Description sample_term_id: NTR_0000853
assay_term_id: OBI_0000716
nucleic_acid_term_id: SO_0000352
Design description: Human Chromatin IP REMC Sequencing on Illumina
Library name: Lib:MC:20101025:02--ChIP:MC:20101020:02:ES cell HUES_64:H3K4Me3
EDACC Genboree Experiment Page:
http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FBroad%2FEXPERIMENT%2FEDACC.5708
EDACC Genboree Sample Page:
http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FBroad%2FSAMPLE%2FEDACC.5931
****************
For data usage terms and conditions, please refer to:
http://www.drugabuse.gov/funding/funding-opportunities/nih-common-fund/epigenomics-data-access-policies
****************
Data processing **********************************************************************

ANALYSIS FILE NAME: GSM669967_BI.HUES64.H3K4me3.Lib_MC_20101025_02--ChIP_MC_20101020_02_ES_cell_HUES_64_H3K4Me3.bed
ANALYSIS CENTER: EDACC
ANALYSIS ALIAS: Lib:MC:20101025:02--ChIP:MC:20101020:02:ES cell HUES_64:H3K4Me3.hg19.level.1.release.3
ANALYSIS TITLE: Mapping of HUES64 Cell Line Histone H3K4me3 ChIP-Seq Data
ANALYSIS DESCRIPTION: Illumina reads produced by Histone H3K4me3 ChIP-Seq on the HUES64 Cell Line, Library Lib:MC:20101025:02--ChIP:MC:20101020:02:ES cell HUES_64:H3K4Me3 were mapped to the human genome using Pash.
ANALYSIS TYPE: REFERENCE_ALIGNMENT
EDACC Genboree Analysis Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FEDACC%2FANALYSIS%2FEDACC.6452
DATA_ANALYSIS_LEVEL: 1
EXPERIMENT_TYPE: ChIP-Seq
GENOME_ASSEMBLY: NCBI Build GRCh37/UCSC Build hg19
SOFTWARE: Pash
SOFTWARE_VERSION: 3.0
MAXIMUM_ALIGNMENT_LENGTH: Read length
MISMATCHES_ALLOWED: 10% of read length
ALIGNMENTS_ALLOWED: 1
TREATMENT_OF_MULTIPLE_ALIGNMENTS: If a read maps to more than 1 position it is removed from consideration.
TREATMENT_OF_IDENTICAL_ALIGNMENTS_OF_MULTIPLE_READS: If multiple reads map to the same start position on the + strand or stop position on the - strand, only a single read is retained.
ALIGNMENT_POSTPROCESSING: None
READ_EXTENSION: 200bp
RELEASE_NUMBER: Human Epigenome Atlas 3


QUALITY SCORES:
NUMBER_OF_H3K4me3_EXPERIMENTS_SCORED_IN_THIS_RELEASE: 63
FINDPEAKS_SCORE: 0.6568
FINDPEAKS_PERCENTILE: 92
HOTSPOT_SCORE: 0.6513
HOTSPOT_PERCENTILE: 92
IROC_SCORE: 0.9983
IROC_PERCENTILE: 44
POISSON_SCORE: 0.6758
POISSON_PERCENTILE: 92

**********************************************************************

ANALYSIS FILE NAME: GSM669967_BI.HUES64.H3K4me3.Lib_MC_20101025_02--ChIP_MC_20101020_02_ES_cell_HUES_64_H3K4Me3.wig
ANALYSIS CENTER: EDACC
ANALYSIS ALIAS: Lib:MC:20101025:02--ChIP:MC:20101020:02:ES cell HUES_64:H3K4Me3.hg19.level.2.release.3
ANALYSIS TITLE: Raw Signal Density Graphs of HUES64 Cell Line Histone H3K4me3 ChIP-Seq Data
ANALYSIS DESCRIPTION: Illumina Histone H3K4me3 ChIP-Seq read mappings from the HUES64 Cell Line, Library Lib:MC:20101025:02--ChIP:MC:20101020:02:ES cell HUES_64:H3K4Me3 were processed into density graphs of raw signal representing the aligned read density.
ANALYSIS TYPE: ABUNDANCE_MEASUREMENT
EDACC Genboree Analysis Page: http://genboree.org/java-bin/project.jsp?projectName=XML%20Submissions%2FEDACC%2FANALYSIS%2FEDACC.6662
DATA_ANALYSIS_LEVEL: 2
EXPERIMENT_TYPE: ChIP-Seq
GENOME_ASSEMBLY: NCBI Build GRCh37/UCSC Build hg19
SOFTWARE: In house programs and scripts
SOFTWARE_VERSION: NA
READ_EXTENSION: 200bp
TREATMENT_OF_IDENTICAL_ALIGNMENTS_OF_MULTIPLE_READS: If multiple reads map to the same start position on the + strand or stop position on the - strand, only a single read is retained.
GENOMIC_WINDOW: 20bp
TREATMENT_OF_REGIONS_PRONE_TO_MULTIPLE_ALIGNMENTS: None
RELEASE_NUMBER: Human Epigenome Atlas 3
BROWSER_TRACK_NAME: HUES64 H3K4me3 e3
BROWSER_TRACK_DESCRIPTION: BI HUES64 Cell Line Histone H3K4me3 Library Lib:MC:20101025:02--ChIP:MC:20101020:02:ES cell HUES_64:H3K4Me3 EA Release 3


QUALITY SCORES:
NUMBER_OF_H3K4me3_EXPERIMENTS_SCORED_IN_THIS_RELEASE: 63
FINDPEAKS_SCORE: 0.6568
FINDPEAKS_PERCENTILE: 92
HOTSPOT_SCORE: 0.6513
HOTSPOT_PERCENTILE: 92
IROC_SCORE: 0.9983
IROC_PERCENTILE: 44
POISSON_SCORE: 0.6758
POISSON_PERCENTILE: 92

**********************************************************************

 
Submission date Feb 07, 2011
Last update date Nov 05, 2019
Contact name BROAD INSTITUTE
E-mail(s) rharris1@bcm.tmc.edu
Organization name Broad Institute
Street address -
City Cambridge
State/province MA
ZIP/Postal code 02142
Country USA
 
Platform ID GPL10999
Series (1)
GSE19465 BI Human Reference Epigenome Mapping Project: ChIP-Seq in human subject
Relations
Reanalyzed by GSE99453
SRA SRX041013
BioSample SAMN00205667
Named Annotation GSM669967_BI.HUES64.H3K4me3.Lib_MC_20101025_02--ChIP_MC_20101020_02_ES_cell_HUES_64_H3K4Me3.wig.gz

Supplementary file Size Download File type/resource
GSM669967_BI.HUES64.H3K4me3.Lib_MC_20101025_02--ChIP_MC_20101020_02_ES_cell_HUES_64_H3K4Me3.bed.gz 339.3 Mb (ftp)(http) BED
GSM669967_BI.HUES64.H3K4me3.Lib_MC_20101025_02--ChIP_MC_20101020_02_ES_cell_HUES_64_H3K4Me3.wig.gz 30.5 Mb (ftp)(http) WIG
SRA Run SelectorHelp
Processed data provided as supplementary file
Raw data are available in SRA

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