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Status |
Public on Jan 26, 2023 |
Title |
border zone, MI-4 |
Sample type |
SRA |
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Source name |
border zone
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Organism |
Mus musculus |
Characteristics |
tissue: border zone genotype: WT treatment: MI time: D7
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Extracted molecule |
total RNA |
Extraction protocol |
RNA was harvested using Rneasy mini plus kit (Qiagen). 1.3 ug of total RNA was used for the construction of sequencing libraries. RNA-seq libraries were constructed by a modified Smartseq2 method with UMI barcodes (Nature 533:487, 2016).
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Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NovaSeq 6000 |
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Data processing |
Sequenced reads were trimmed for adaptor sequence, and then mapped to mm10 mouse genome using STAR. Aligned reads with high quality were quantified by HTSeq. Counts per million reads (CPM) were calculated. Unique molecular identifiers (UMIs) were extracted from Read2. Raw gene counts were calculated by UMI number to get rmdup.count file. Assembly: mm10 Supplementary files format and content: tab-delimited text files include counts for each Sample
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Submission date |
Oct 25, 2022 |
Last update date |
Jan 28, 2023 |
Contact name |
Dandan Cao |
E-mail(s) |
1931465@tongji.edu.cn
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Phone |
17853136996
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Organization name |
Tongji University
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Street address |
1239 Siping Road, Yangpu District
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City |
Shanghai |
ZIP/Postal code |
200092 |
Country |
China |
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Platform ID |
GPL24247 |
Series (1) |
GSE184986 |
Inhibition of Fap Promotes Cardiac Repair by Stabilizing BNP |
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Relations |
BioSample |
SAMN31438029 |
SRA |
SRX18007819 |
Supplementary file |
Size |
Download |
File type/resource |
GSM6675448_MI-4.count.txt.gz |
210.9 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
Raw data are available in SRA |
Processed data provided as supplementary file |
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