|
Status |
Public on May 15, 2023 |
Title |
cSiah1a/2f/f::Lyz2cre alveolar macrophage, replicate 3 |
Sample type |
SRA |
|
|
Source name |
lung
|
Organism |
Mus musculus |
Characteristics |
tissue: lung cell type: alveolar macrophage genotype: cSiah1a/2f/f::Lyz2cre
|
Extracted molecule |
total RNA |
Extraction protocol |
PolyA RNA was isolated using the NEBNext® Poly(A) mRNA Magnetic Isolation Module. Barcoded libraries were constructed using the NEBNext® UltraTM Directional RNA Library Prep Kit for Illumina (NEB, Ipswich MA). Libraries were pooled and sequenced single-end (1X75) on an Illumina NextSeq 500 using the High output V2 kit (Illumina Inc., San Diego CA).
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina NextSeq 500 |
|
|
Description |
LyzS_rep3
|
Data processing |
Illumina Truseq adapters and polyA/polyT sequences were trimmed from raw reads using Cutadapt version 2.3. Trimmed reads were aligned to mouse genome version mm10 and Ensembl gene annotations version 84 using STAR version 2.7.0d_0221 adopting alignment parameters from ENCODE long RNA-seq pipeline (https://github.com/ENCODE-DCC/long-rna-seq-pipeline). RSEM version 1.3.1 was used to obtain gene level estimated counts and transcripts per million (TPM). Assembly: mm10 Supplementary files format and content: Tab-delimited text file includes TPM (Transcripts Per Million) values for each sample.
|
|
|
Submission date |
Oct 17, 2022 |
Last update date |
May 15, 2023 |
Contact name |
Ze'ev A. Ronai |
E-mail(s) |
ronai@sbpdiscovery.org
|
Organization name |
Sanford Burnham Prebys Medical Discovery Institute
|
Department |
Tumor Initiation and Maintenance Program, NCI-Designated Cancer Center
|
Street address |
10901 North Torrey Pines Road
|
City |
La Jolla |
State/province |
CA |
ZIP/Postal code |
92037 |
Country |
USA |
|
|
Platform ID |
GPL19057 |
Series (2) |
GSE215920 |
Control of alveolar macrophage differentiation by Siah1a/2 ubiquitin ligases limits carcinogen-induced lung adenocarcinoma [Bulk RNA-seq] |
GSE215993 |
Control of alveolar macrophage differentiation by Siah1a/2 ubiquitin ligases limits carcinogen-induced lung adenocarcinoma |
|
Relations |
BioSample |
SAMN31318059 |
SRA |
SRX17916147 |