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| Status |
Public on Jan 17, 2023 |
| Title |
Lung,10,rep4 |
| Sample type |
SRA |
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| Source name |
Lung
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| Organism |
Homo sapiens |
| Characteristics |
tissue: Lung
age: 10
donor: XDD371
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| Extracted molecule |
total RNA |
| Extraction protocol |
One of the two lungs (preferentially the left), from each donor, was snap frozen in cryomatrix and further used for histological analyses. We cut 10-12μm-thick tissue sections with a cryostat (Leica CM3050S or analogue) and collected them onto poly-lysine coated slides (VWR Cat No. 631-0107)
Spatial Gene Expression libraries (n=9) (6-13 pcw) were generated with the Visium Spatial Gene Expression Slide & Reagent kit (PN-1000184; 10X Genomics Inc.), according to manufacturer's protocol
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| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
NextSeq 2000 |
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| Description |
10X Genomics
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| Data processing |
Sequenced ST libraries were processed using Space Ranger 1.0.0 Pipeline (10X Genomics).
Reads were aligned to the human reference genome to obtain an expression matrix.
Assembly: GRCh38-3.0.0
Supplementary files format and content: Matrix tables with raw gene counts for every gene and every sample
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| Submission date |
Oct 17, 2022 |
| Last update date |
Jan 17, 2023 |
| Contact name |
Paulo Czarnewski |
| E-mail(s) |
czarnewski@gmail.com
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| Organization name |
KTH
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| Street address |
Tomtebodavägen 23b
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| City |
Solna |
| ZIP/Postal code |
171 65 |
| Country |
Sweden |
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| Platform ID |
GPL30173 |
| Series (2) |
| GSE215897 |
A topographic atlas defines developmental origins of cell heterogeneity in the human embryonic lung [ST] |
| GSE215898 |
A topographic atlas defines developmental origins of cell heterogeneity in the human embryonic lung |
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| Supplementary file |
Size |
Download |
File type/resource |
| GSM6645182_V10F24_076_D1.tar.gz |
143.0 Mb |
(ftp)(http) |
TAR |
| Processed data provided as supplementary file |
| Raw data not provided for this record |
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