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Sample GSM6645169 Query DataSets for GSM6645169
Status Public on Jan 17, 2023
Title Lung,7,rep3
Sample type SRA
 
Source name Lung
Organism Homo sapiens
Characteristics tissue: Lung
age: 7
donor: XDD399
Extracted molecule total RNA
Extraction protocol One of the two lungs (preferentially the left), from each donor, was snap frozen in cryomatrix and further used for histological analyses. We cut 10-12μm-thick tissue sections with a cryostat (Leica CM3050S or analogue) and collected them onto poly-lysine coated slides (VWR Cat No. 631-0107)
Spatial Gene Expression libraries (n=9) (6-13 pcw) were generated with the Visium Spatial Gene Expression Slide & Reagent kit (PN-1000184; 10X Genomics Inc.), according to manufacturer's protocol
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model NextSeq 2000
 
Description 10X Genomics
Data processing Sequenced ST libraries were processed using Space Ranger 1.0.0 Pipeline (10X Genomics).
Reads were aligned to the human reference genome to obtain an expression matrix.
Assembly: GRCh38-3.0.0
Supplementary files format and content: Matrix tables with raw gene counts for every gene and every sample
 
Submission date Oct 17, 2022
Last update date Jan 17, 2023
Contact name Paulo Czarnewski
E-mail(s) czarnewski@gmail.com
Organization name KTH
Street address Tomtebodavägen 23b
City Solna
ZIP/Postal code 171 65
Country Sweden
 
Platform ID GPL30173
Series (2)
GSE215897 A topographic atlas defines developmental origins of cell heterogeneity in the human embryonic lung [ST]
GSE215898 A topographic atlas defines developmental origins of cell heterogeneity in the human embryonic lung

Supplementary file Size Download File type/resource
GSM6645169_V10F24_108_A1.tar.gz 34.2 Mb (ftp)(http) TAR
Processed data provided as supplementary file
Raw data not provided for this record

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