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Sample GSM6616343 Query DataSets for GSM6616343
Status Public on May 11, 2023
Title MEF, DKO_A1, ctrl, rep2
Sample type SRA
 
Source name mouse embryonic fibroblast
Organism Mus musculus
Characteristics cell type: mouse embryonic fibroblast
genotype: LKB1/CRTC2 knockout clone A1
treatment: control
Extracted molecule total RNA
Extraction protocol RNA was harvested using Rneasy mini kit (Qiagen). 1 ug of total RNA was used for the construction of sequencing libraries.
RNA libraries for RNA-seq were prepared using SMARTER mRNA-Seq Library Prep Kit following manufacturer's protocols.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Data processing Reads were trimmed with TrimGalore v0.6.0.
Using STAR v2.7.8a, trimmed reads were aligned to mm10 GENCODE vM24 to obtain read counts per gene.
Assembly: mm10
Supplementary files format and content: tab-delimited file containing read counts per gene.
 
Submission date Oct 04, 2022
Last update date May 11, 2023
Contact name Russell Jones
E-mail(s) russell.jones@vai.org
Phone 6166001610
Organization name Van Andel Institute
Street address 333 Bostwick Ave. NE
City Grand Rapids
State/province MI
ZIP/Postal code 49503
Country USA
 
Platform ID GPL24247
Series (2)
GSE214823 RNA-seq profiling of WT, LKB1 KO, and LKB1/CRTC2 KO mouse embryonic fibroblasts
GSE214824 WT, LKB1 KO, and LKB1/CRTC2 KO mouse embryonic fibroblasts
Relations
BioSample SAMN31155883
SRA SRX17797905

Supplementary file Size Download File type/resource
GSM6616343_SC13.ReadsPerGene.out.tab.gz 342.7 Kb (ftp)(http) TAB
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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