|
| Status |
Public on Mar 15, 2011 |
| Title |
HeLa cells, replicate 1 of 2 |
| Sample type |
RNA |
| |
|
| Source name |
HeLa cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HeLa
|
| Treatment protocol |
RNA were harvest before or after TNF-alpha treatment (10ng/mL) for 2hr
|
| Growth protocol |
HeLa and THP-1 cells were obtained from ATCC and cultured under recommended conditions.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Affymetrix protocol
|
| |
|
| Hybridization protocol |
Affymetrix protocol
|
| Scan protocol |
Affymetrix protocol
|
| Description |
Gene expression data for untreated HeLa cells.
|
| Data processing |
GC-RMA normalization using GCRMA package (version 2.14.1) in R
|
| |
|
| Submission date |
Jan 25, 2011 |
| Last update date |
Mar 15, 2011 |
| Contact name |
Fulai Jin |
| E-mail(s) |
fxj45@case.edu
|
| Phone |
2163681811
|
| Organization name |
Case Western Reserve University
|
| Lab |
Fulai Jin
|
| Street address |
10900 Euclid Avenue
|
| City |
Cleveland |
| State/province |
OH - OHIO |
| ZIP/Postal code |
44106 |
| Country |
USA |
| |
|
| Platform ID |
GPL570 |
| Series (2) |
| GSE26868 |
PU.1 and C/EBPalpha synergistically program distinct response to NF-kappaB activation through establishing monocyte specific enhancers (expression data) |
| GSE26994 |
PU.1 and C/EBPalpha synergistically program distinct response to NF-kappaB activation through establishing monocyte-specific enhancers |
|
