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Sample GSM661187 Query DataSets for GSM661187
Status Public on Sep 18, 2012
Title H1_rep2
Sample type RNA
 
Source name H1 embryonic stem cells
Organism Homo sapiens
Characteristics treatment: none
cell type: H1 embryonic stem cells
cell line: H1
Treatment protocol Human keratinocytes were treated with 1.5 mM of calcium chloride for 48 hours.
Growth protocol IMR90 human primary lung embryo fibroblasts (ATCC) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) plus 10% FBS (Hyclone), 100 U/ml penicillin (Gibco), and 100 µg/ml streptomycin (Gibco) at 37°C in 5% CO2. Growing cells with 50~70% confluence were used for further analysis.
Extracted molecule total RNA
Extraction protocol ~200 ng of total RNA were extracted from ~50 million cells by using Trizol (Invitrogen) according to manufacturer’s instructions, and further purified by RNeasy Plus Mini Kit (QiaGen). cDNA libraries were prepared by using SuperScript III First-strand Synthesis SuperMix with random haxamers (Invitrogen).
Label Cy3
Label protocol Synthesized cDNAs were amplified and labelled with Cy3 (Perkin Elmer) by using Agilent One Colour Low RNA Input Linear Amplification Labelling Kit according to manufacturer’s instructions.
 
Hybridization protocol Labelled cDNAs were hybridized to the Agilent Human Whole-genome array (G2534-60011) as per manufacturer's instructions.
Scan protocol standard Agilent protocol
Description biological replicate 2 of 2
Data processing standard Agilent protocol
 
Submission date Jan 25, 2011
Last update date Sep 18, 2012
Contact name Jing-Yu Li
E-mail(s) jingyuli@mednet.ucla.edu
Organization name University of California, Los Angeles
Street address 615 Charles E. Young Dr. South BSRB 357
City Los Angeles
ZIP/Postal code 90095
Country USA
 
Platform ID GPL4133
Series (2)
GSE26855 Gene expression profile of H1 human embryonic stem cells, IMR90 lung fibroblasts, calcium induced keratinocytes, and primary hepatocytes
GSE26979 Human Linker Histone H1.5

Data table header descriptions
ID_REF
VALUE Intensities from all samples were quantile normalized.

Data table
ID_REF VALUE
556 null
775 null
824 null
1408 null
1778 null
2154 null
2240 null
2370 null
2391 null
2911 null
3154 null
3270 null
3350 null
4064 null
4080 null
4481 null
4641 null
4686 null
6457 null
6480 null

Total number of rows: 45015

Table truncated, full table size 695 Kbytes.




Supplementary file Size Download File type/resource
GSM661187_US23502366_251485019067_S01_2.txt.gz 8.7 Mb (ftp)(http) TXT
Processed data included within Sample table

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