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Sample GSM658295 Query DataSets for GSM658295
Status Public on Jan 21, 2011
Title normal_4
Sample type RNA
 
Source name normal Patient 4
Organism Homo sapiens
Characteristics gender: male
tissue: normal prostate
cell-type: dendritic cells
Treatment protocol Cells were purified from digested prostates by magnetic beads >90% purity was confirmed
Extracted molecule total RNA
Extraction protocol RNA was isolated using the qiagen RNAeasy mini spin colomn according to manufacturer's instuctions. All samples used for microarray analysis have high quality score (RIN >9).
RNA quality was checked on Agilent Bioanalyzer
Label biotin
Label protocol 200 ng of RNA was reverse transcribed with random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol
 
Hybridization protocol replicates of each group were hybridized to Affymetrixhuman Gene ST 1.0 GeneChip in Affymetrix hybridization oven at 45C, 60rpm for 16 hrs
Scan protocol Wash and stain were performed on Affymetrix Fluidics Station 450 and scanned on Affymetrix GeneChip scanner 3000
Description Dendritic cells normal human prostate patient 4
Data processing Data were collected using Affymetrix AGCC software,Statistical and clustering analysis was performed with Partek Genomics Suite software using RMA normalization algorithm.
 
Submission date Jan 20, 2011
Last update date Jan 21, 2011
Contact name Stephanie Kaye Watkins
E-mail(s) watkinssk@mail.nih.gov
Phone 301-846-6871
Fax 301-846-6005
Organization name NCI-Frederick
Department CIP/LMI
Lab Tumor Immunity and Tolerance
Street address 1050 Boyles St.
City Frederick
State/province MD
ZIP/Postal code 21702
Country USA
 
Platform ID GPL10739
Series (1)
GSE26747 Gene expression analysis of dendritic cells from normal or tumor sections of human prostates

Data table header descriptions
ID_REF Affymetrix ID
VALUE Log2 RMA signal intensity

Data table
ID_REF VALUE
7892501 2.13317
7892502 3.47951
7892503 2.32453
7892504 7.79876
7892505 2.68002
7892506 2.46027
7892507 4.78811
7892508 3.09421
7892509 7.85283
7892510 2.77579
7892511 2.01448
7892512 7.07806
7892513 3.18688
7892514 6.55984
7892515 8.22101
7892516 2.81359
7892517 3.24291
7892518 2.5959
7892519 6.15601
7892520 9.76651

Total number of rows: 257101

Table truncated, full table size 3992 Kbytes.




Supplementary file Size Download File type/resource
GSM658295.CEL.gz 3.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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