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Sample GSM658290 Query DataSets for GSM658290
Status Public on Jan 21, 2011
Title tumor_4
Sample type RNA
 
Source name Tumor Patient 4
Organism Homo sapiens
Characteristics gender: male
tissue: tumor bearing prostate
cell-type: dendritic cells
Treatment protocol Cells were purified from digested prostates by magnetic beads >90% purity was confirmed
Extracted molecule total RNA
Extraction protocol RNA was isolated using the qiagen RNAeasy mini spin colomn according to manufacturer's instuctions. All samples used for microarray analysis have high quality score (RIN >9).
RNA quality was checked on Agilent Bioanalyzer
Label biotin
Label protocol 200 ng of RNA was reverse transcribed with random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol
 
Hybridization protocol replicates of each group were hybridized to Affymetrixhuman Gene ST 1.0 GeneChip in Affymetrix hybridization oven at 45C, 60rpm for 16 hrs
Scan protocol Wash and stain were performed on Affymetrix Fluidics Station 450 and scanned on Affymetrix GeneChip scanner 3000
Description Dendritic cells tumor human prostate patient 4
Data processing Data were collected using Affymetrix AGCC software,Statistical and clustering analysis was performed with Partek Genomics Suite software using RMA normalization algorithm.
 
Submission date Jan 20, 2011
Last update date Jan 21, 2011
Contact name Stephanie Kaye Watkins
E-mail(s) watkinssk@mail.nih.gov
Phone 301-846-6871
Fax 301-846-6005
Organization name NCI-Frederick
Department CIP/LMI
Lab Tumor Immunity and Tolerance
Street address 1050 Boyles St.
City Frederick
State/province MD
ZIP/Postal code 21702
Country USA
 
Platform ID GPL10739
Series (1)
GSE26747 Gene expression analysis of dendritic cells from normal or tumor sections of human prostates

Data table header descriptions
ID_REF Affymetrix ID
VALUE Log2 RMA signal intensity

Data table
ID_REF VALUE
7892501 2.07912
7892502 3.5126
7892503 3.07567
7892504 7.85621
7892505 2.62236
7892506 3.21522
7892507 3.73124
7892508 3.45898
7892509 6.51356
7892510 2.5809
7892511 2.36339
7892512 6.93764
7892513 3.02981
7892514 5.4726
7892515 8.86685
7892516 2.68078
7892517 2.95361
7892518 2.80683
7892519 6.10993
7892520 9.89146

Total number of rows: 257101

Table truncated, full table size 3990 Kbytes.




Supplementary file Size Download File type/resource
GSM658290.CEL.gz 3.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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