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Sample GSM658289 Query DataSets for GSM658289
Status Public on Jan 21, 2011
Title tumor_3
Sample type RNA
 
Source name Tumor Patient 3
Organism Homo sapiens
Characteristics gender: male
tissue: tumor bearing prostate
cell-type: dendritic cells
Treatment protocol Cells were purified from digested prostates by magnetic beads >90% purity was confirmed
Extracted molecule total RNA
Extraction protocol RNA was isolated using the qiagen RNAeasy mini spin colomn according to manufacturer's instuctions. All samples used for microarray analysis have high quality score (RIN >9).
RNA quality was checked on Agilent Bioanalyzer
Label biotin
Label protocol 200 ng of RNA was reverse transcribed with random hexamer primer, amplified and terminal labeled with biotin using Affymetrix Whole Transcript Sense Target Labeling kit according to manufacturer’s protocol
 
Hybridization protocol replicates of each group were hybridized to Affymetrixhuman Gene ST 1.0 GeneChip in Affymetrix hybridization oven at 45C, 60rpm for 16 hrs
Scan protocol Wash and stain were performed on Affymetrix Fluidics Station 450 and scanned on Affymetrix GeneChip scanner 3000
Description Dendritic cells tumor human prostate patient 3
Data processing Data were collected using Affymetrix AGCC software,Statistical and clustering analysis was performed with Partek Genomics Suite software using RMA normalization algorithm.
 
Submission date Jan 20, 2011
Last update date Jan 21, 2011
Contact name Stephanie Kaye Watkins
E-mail(s) watkinssk@mail.nih.gov
Phone 301-846-6871
Fax 301-846-6005
Organization name NCI-Frederick
Department CIP/LMI
Lab Tumor Immunity and Tolerance
Street address 1050 Boyles St.
City Frederick
State/province MD
ZIP/Postal code 21702
Country USA
 
Platform ID GPL10739
Series (1)
GSE26747 Gene expression analysis of dendritic cells from normal or tumor sections of human prostates

Data table header descriptions
ID_REF Affymetrix ID
VALUE Log2 RMA signal intensity

Data table
ID_REF VALUE
7892501 2.04195
7892502 3.50172
7892503 3.22151
7892504 7.64612
7892505 2.79425
7892506 2.64732
7892507 4.21224
7892508 3.56577
7892509 6.70992
7892510 2.50962
7892511 2.06856
7892512 6.92328
7892513 2.85783
7892514 5.1138
7892515 8.64201
7892516 2.68983
7892517 3.13887
7892518 2.4054
7892519 6.14871
7892520 9.60639

Total number of rows: 257101

Table truncated, full table size 3993 Kbytes.




Supplementary file Size Download File type/resource
GSM658289.CEL.gz 3.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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