|
Status |
Public on Sep 13, 2005 |
Title |
DB4_925 mouse breast tumor |
Sample type |
genomic |
|
|
Channel 1 |
Source name |
Mouse breast tumor
|
Organism |
Mus musculus |
Characteristics |
Mouse strain FVB/N
|
Biomaterial provider |
William J Muller
|
Treatment protocol |
|
Growth protocol |
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Hodgson et al.(2001) Nat Genet, 29, 459-64.
|
Label |
Cy3
|
Label protocol |
Hodgson et al.(2001) Nat Genet, 29, 459-64.
|
|
|
Channel 2 |
Source name |
Normal mouse spleen
|
Organism |
Mus musculus |
Characteristics |
Mouse strain FVB/N
|
Biomaterial provider |
J Graeme Hodgson
|
Treatment protocol |
|
Growth protocol |
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Hodgson et al.(2001) Nat Genet, 29, 459-64.
|
Label |
Cy5
|
Label protocol |
Hodgson et al.(2001) Nat Genet, 29, 459-64.
|
|
|
|
Hybridization protocol |
Hodgson et al.(2001) Nat Genet, 29, 459-64.
|
Scan protocol |
Hodgson et al.(2001) Nat Genet, 29, 459-64.
|
Description |
Mouse breast tumor
|
Data processing |
Hodgson et al.(2001) Nat Genet, 29, 459-64.
|
|
|
Submission date |
Jul 25, 2005 |
Last update date |
Jul 27, 2005 |
Contact name |
Graeme Hodgson |
E-mail(s) |
ghodgson@cc.ucsf.edu
|
Phone |
415-476-3630
|
Fax |
415-476-8218
|
Organization name |
UCSF
|
Department |
Neurological Surgery
|
Street address |
Box0808
|
City |
San Francisco |
State/province |
CA |
ZIP/Postal code |
94143 |
Country |
USA |
|
|
Platform ID |
GPL2642 |
Series (1) |
GSE3012 |
Copy number aberrations in mouse breast tumors reveal loci and genes important in tumorigenic RTK signaling |
|
Data table header descriptions |
ID_REF |
|
SpotNum |
Unique integer for each spot. |
Image-x |
X image coordinate of spot center. |
Image-y |
Y image coordinate of spot center. |
Arr-colx |
Subgrid column (1-based). |
Arr-rowy |
Subgrid row. |
Spot-colx |
Spot column (within subgrid). |
Spot-rowy |
Spot row. |
Flag |
Flag: 0 if spot is OK. |
nfore |
Number of foreground pixels. |
nback |
Number of background pixels. |
DapiFore |
Dapi (or composite) channel foreground mean. |
DapiBack |
Dapi background mean. |
Dapi |
Dapi signal (fore - back). |
TestFore |
Test foreground mean. |
TestForeSD |
Test foreground standard deviation. |
TestBack |
Test background mean. |
TestBackSD |
Test background standard deviation. |
Test |
Test signal (fore - back). |
RefFore |
Reference foreground mean. |
RefForeSD |
Reference foreground standard deviation. |
RefBack |
Reference background mean. |
RefBackSD |
Reference background standard deviation. |
Ref |
Reference signal (fore - back). |
VALUE |
log2(RawRat) |
RawRat |
Test/Ref |
SpotCorr |
Pearson's correlation of test to ref foreground pixels. |
TestZstat |
Z-statistic of difference between test fore and back. |
RefZstat |
Z-statistic of difference between ref fore and back. |
MinF-B |
Minimum (fore-back) for all three channels. |
Slope |
Slope of line fit to test/ref fore pixels |
Log2Slope |
log2(Slope) |
TestIntercept |
Normalized intercept. |
RefIntercept |
Normalized intercept. |
OriginDist |
Distance of intercept to origin. |
MeanLog2Rat |
Mean of pixel by pixel log2(ratio) |
MeanRat |
Corresponding non-logged ratio to MeanLog2Rat |
MedianRatio |
Median pixel by pixel ratio. |
Log2MedRat |
log2(MedianRatio) |