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Sample GSM651370 Query DataSets for GSM651370
Status Public on Jul 20, 2011
Title wild type +3AT 111A-2
Sample type SRA
Source name wild type +3AT
Organism Saccharomyces cerevisiae
Characteristics strain: YDC111 (W303)
genotype: MATa ade2-1 can1-100 leu2-3,112 trp1-1 ura3-1
average length (bp) +/-sd: 153.2 +/- 9.3
assayed molecule: nucleosomal DNA
Treatment protocol Nuclei were prepared and digested to mono-nucleosomes with micrococcal nuclease. Mono-nucleosomal DNA was gel-purified.
Growth protocol Cells grown to mid-log phase (OD600 about 0.8) at 30 degC in synthetic complete medium (control), or in synthetic complete medium lacking histidine, with 10 mM 3-aminotriazole for 20 mins prior to harvesting (+3AT).
Extracted molecule genomic DNA
Extraction protocol The DNA was repaired using the DNA repair kit from New England Biolabs. The repaired DNA was processed for paired-end sequencing according to the Illumina protocol.
Library strategy MNase-Seq
Library source genomic
Library selection MNase
Instrument model Illumina Genome Analyzer II
Data processing ELAND was used to align the reads to the NCBI Saccharomyces cerevisiae genome sequence Build 2.1
Submission date Jan 07, 2011
Last update date May 15, 2019
Contact name David Johannes Clark
Phone 3014966966
Organization name NICHD, NIH
Department DDB
Street address 6 Center Drive Bldg 6A Rm 2A02
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platform ID GPL9377
Series (1)
GSE26493 Genome-wide nucleosome position maps in Saccharomyces cerevisiae
SRA SRX038809
BioSample SAMN00191467

Supplementary file Size Download File type/resource
GSM651370_111A-2_s_7_1_sorted.txt.gz 282.3 Mb (ftp)(http) TXT
GSM651370_111A-2_s_7_2_sorted.txt.gz 286.2 Mb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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