|
Status |
Public on Jul 20, 2011 |
Title |
wild type control 111U-3 |
Sample type |
SRA |
|
|
Source name |
wild type control
|
Organism |
Saccharomyces cerevisiae |
Characteristics |
strain: YDC111 (W303) genotype: MATa ade2-1 can1-100 leu2-3,112 trp1-1 ura3-1 average length (bp) +/-sd: 156.0 +/- 11.3 assayed molecule: nucleosomal DNA
|
Treatment protocol |
Nuclei were prepared and digested to mono-nucleosomes with micrococcal nuclease. Mono-nucleosomal DNA was gel-purified.
|
Growth protocol |
Cells grown to mid-log phase (OD600 about 0.8) at 30 degC in synthetic complete medium (control), or in synthetic complete medium lacking histidine, with 10 mM 3-aminotriazole for 20 mins prior to harvesting (+3AT).
|
Extracted molecule |
genomic DNA |
Extraction protocol |
The DNA was repaired using the DNA repair kit from New England Biolabs. The repaired DNA was processed for paired-end sequencing according to the Illumina protocol.
|
|
|
Library strategy |
MNase-Seq |
Library source |
genomic |
Library selection |
MNase |
Instrument model |
Illumina Genome Analyzer II |
|
|
Data processing |
ELAND was used to align the reads to the NCBI Saccharomyces cerevisiae genome sequence Build 2.1
|
|
|
Submission date |
Jan 07, 2011 |
Last update date |
May 15, 2019 |
Contact name |
David Johannes Clark |
E-mail(s) |
clarkda@mail.nih.gov
|
Phone |
3014966966
|
Organization name |
NICHD, NIH
|
Department |
DDB
|
Lab |
SCGE
|
Street address |
6 Center Drive Bldg 6A Rm 2A02
|
City |
Bethesda |
State/province |
MD |
ZIP/Postal code |
20892 |
Country |
USA |
|
|
Platform ID |
GPL9377 |
Series (1) |
GSE26493 |
Genome-wide nucleosome position maps in Saccharomyces cerevisiae |
|
Relations |
SRA |
SRX038808 |
BioSample |
SAMN00191466 |