|
| Status |
Public on Aug 29, 2022 |
| Title |
S1-1 |
| Sample type |
SRA |
| |
|
| Source name |
flowers
|
| Organism |
Brassica juncea |
| Characteristics |
cell type: flowers
genotype: Apetalous Nullisomic Brassica juncea "AN"
treatment: Formation of apetalous mechanism
|
| Extracted molecule |
total RNA |
| Extraction protocol |
The flowers were removed, flash frozen on dry ice, and RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
AN_1
|
| Data processing |
Illumina software used for basecalling
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to SCHZ genome using HISAT2
The FPKM (Fragments per Kilobase of transcript per Million mapped reads) was calculated
The GO annotations for the DEGs in leaf were determined by using Blast2GO
Assembly: GCA_018703725.1
Supplementary files format and content: Matrix table with raw gene counts for every gene and every sample
|
| |
|
| Submission date |
Aug 26, 2022 |
| Last update date |
Aug 29, 2022 |
| Contact name |
jie yu |
| E-mail(s) |
yujiehzs@126.com
|
| Phone |
18208671213
|
| Organization name |
Guizhou Normal Universuty
|
| Street address |
116 Baoshan North Road
|
| City |
Guiyang |
| ZIP/Postal code |
550000 |
| Country |
China |
| |
|
| Platform ID |
GPL32601 |
| Series (1) |
| GSE212102 |
Development and characteristics of an apetalous nullisomic in Brassica juncea through interspecific hybridization |
|
| Relations |
| BioSample |
SAMN30521937 |
| SRA |
SRX17220314 |
