|
| Status |
Public on Aug 26, 2022 |
| Title |
RNA-Seq of mus musculus:adult male brain (CBSST-Seq) |
| Sample type |
SRA |
| |
|
| Source name |
Brain
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Brain
Sex: Male
age: 10 weeks
strain: C57BL/6J
treatment: Cryosection
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Tissue was permeabilized with tissue permeabilizing enzyme for 12 minutes
RNA libraries for RNA-seq were prepared using Kapa HIFI HotStart ReadyMix PCR Kit following manufacturer's protocols.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
CBSST-Seq
|
| Data processing |
The filtered data was mapped to the mouse genome (mm10 mouse) using SpaceRanger V1.1.0.
Assembly: mm10
Supplementary files format and content: Tab-separated values files and matrix files
|
| |
|
| Submission date |
Aug 23, 2022 |
| Last update date |
Sep 22, 2022 |
| Contact name |
Zhengyang Jin |
| E-mail(s) |
jinzy21@mails.jlu.edu.cn
|
| Organization name |
Jilin University
|
| Street address |
No. 1163 Xinmin Street
|
| City |
Changchun |
| State/province |
Jilin |
| ZIP/Postal code |
130021 |
| Country |
China |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE211858 |
Cross-amplified Barcodes on Slides for Spatial Transcriptomics Sequencing |
|
| Relations |
| SRA |
SRX17158482 |
| BioSample |
SAMN30418579 |
