Biopsied trophectoderm and arrested whole embryo samples underwent alkaline lysis for genomic DNA extraction as described previously (Cui et al., 1989; PNAS 86, 9389-93).
Label
biotin
Label protocol
DNA was digested with the NspI restriction enzyme, ligated to an adapter molecule, PCR amplified, fragmented with DNase I, and biotinylated according to the manufacturer's recommendations (Affymetrix Inc., GeneChip Mapping 500K Assay Manual # 701930).
Hybridization protocol
DNA was hybridized to each array according to the manufacturer's instructions (Affymetrix Inc., GeneChip Mapping 500K Assay Manual # 701930).
Scan protocol
Each array was washed using Affymetrix Fluidics Station 640, and scanned using the GeneChip Scanner 7G as recommended (Affymetrix Inc., GeneChip Mapping 500K Assay Manual # 701930).
Description
Hybridized to 250K_Nsp
Data processing
CEL files were processed using GTYPE version 4 (Affymetrix Inc., Genotyping Console 4.0 Manual) using the DM algorithm for genotype calls.