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Sample GSM647639 Query DataSets for GSM647639
Status Public on Feb 21, 2011
Title Xenopus_xSGK1-morpholinos_rep2
Sample type RNA
Source name xSGK1-morpholinos-injected Xenopus laevis embryos
Organism Xenopus laevis
Characteristics tissue: whole embryo
developmental stage: stage 12
treatment: xSGK1 morpholinos
Treatment protocol We radially injected control-morpholino (80 ng) or xSGK1-morpholinos (morpholino1 40 ng and morpholino2 40 ng) into 4-cell embryos, and cultured the embryos until stage 12.
Growth protocol Xenopus eggs were obtained by in vitro fertilization and were dejellied in 2% cysteine, pH 7.8.
Extracted molecule total RNA
Extraction protocol Total RNA was prepared using TRIzol (Invitrogen) and purified through RNeasy columns (Qiagen), according to the manufacturers' instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the Affymetrix protocol (Two-Cycle Target Labeling Assays) from 100 ng total RNA.
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45 degrees on the GeneChip Xenopus laevis Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using an Affymetrix GeneChip Scanner.
Description xSGK1-MO2
Xenopus embryos injected with xSGK1-morpholinos.
Data processing The data were analyzed with the MAS 5.0 algorithm using GeneChip Operating Software (GCOS) v. 1.4.
Submission date Dec 30, 2010
Last update date Feb 21, 2011
Contact name Eisuke Nishida
Phone +81-75-753-4230
Organization name Graduate School of Biostudies, Kyoto University
Department Department of Cell and Developmental Biology
Street address Kitashirakawa, Sakyo-ku
City Kyoto
ZIP/Postal code 606-8502
Country Japan
Platform ID GPL1318
Series (1)
GSE26381 The Kinase SGK1 in the Endoderm and Mesoderm Promotes Ectodermal Survival by Downregulating Components of the Death-Inducing Signaling Complex

Data table header descriptions
VALUE MAS5.0 signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), or marginal (M)

Data table
AFFX-BioB-5_at 129.3 P
AFFX-BioB-M_at 180.9 P
AFFX-BioB-3_at 123.6 P
AFFX-BioC-5_at 514.3 P
AFFX-BioC-3_at 394.4 P
AFFX-BioDn-5_at 966.2 P
AFFX-BioDn-3_at 1737.2 P
AFFX-CreX-5_at 5165.7 P
AFFX-CreX-3_at 8601 P
AFFX-DapX-5_at 14.2 A
AFFX-DapX-M_at 16.3 A
AFFX-DapX-3_at 3.8 A
AFFX-LysX-5_at 1.7 A
AFFX-LysX-M_at 5.9 A
AFFX-LysX-3_at 18.6 P
AFFX-PheX-5_at 1.4 A
AFFX-PheX-M_at 2.1 A
AFFX-PheX-3_at 17.6 A
AFFX-ThrX-5_at 1.8 A
AFFX-ThrX-M_at 3 A

Total number of rows: 15611

Table truncated, full table size 368 Kbytes.

Supplementary file Size Download File type/resource
GSM647639.CEL.gz 2.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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