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Sample GSM647638 Query DataSets for GSM647638
Status Public on Feb 21, 2011
Title Xenopus_control-morpholino_rep2
Sample type RNA
 
Source name control-morpholino-injected Xenopus laevis embryos
Organism Xenopus laevis
Characteristics tissue: whole embryo
developmental stage: stage 12
treatment: control morpholino
Treatment protocol We radially injected control-morpholino (80 ng) or xSGK1-morpholinos (morpholino1 40 ng and morpholino2 40 ng) into 4-cell embryos, and cultured the embryos until stage 12.
Growth protocol Xenopus eggs were obtained by in vitro fertilization and were dejellied in 2% cysteine, pH 7.8.
Extracted molecule total RNA
Extraction protocol Total RNA was prepared using TRIzol (Invitrogen) and purified through RNeasy columns (Qiagen), according to the manufacturers' instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the Affymetrix protocol (Two-Cycle Target Labeling Assays) from 100 ng total RNA.
 
Hybridization protocol Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45 degrees on the GeneChip Xenopus laevis Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using an Affymetrix GeneChip Scanner.
Description control-MO2
Xenopus embryos injected with control-morpholino.
Data processing The data were analyzed with the MAS 5.0 algorithm using GeneChip Operating Software (GCOS) v. 1.4.
 
Submission date Dec 30, 2010
Last update date Feb 21, 2011
Contact name Eisuke Nishida
E-mail(s) nishida@lif.kyoto-u.ac.jp
Phone +81-75-753-4230
Organization name Graduate School of Biostudies, Kyoto University
Department Department of Cell and Developmental Biology
Street address Kitashirakawa, Sakyo-ku
City Kyoto
ZIP/Postal code 606-8502
Country Japan
 
Platform ID GPL1318
Series (1)
GSE26381 The Kinase SGK1 in the Endoderm and Mesoderm Promotes Ectodermal Survival by Downregulating Components of the Death-Inducing Signaling Complex

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), or marginal (M)

Data table
ID_REF VALUE ABS_CALL
AFFX-BioB-5_at 109.8 P
AFFX-BioB-M_at 199.4 P
AFFX-BioB-3_at 121.3 P
AFFX-BioC-5_at 472.5 P
AFFX-BioC-3_at 518.8 P
AFFX-BioDn-5_at 1006.4 P
AFFX-BioDn-3_at 1643.5 P
AFFX-CreX-5_at 5304.1 P
AFFX-CreX-3_at 9517.5 P
AFFX-DapX-5_at 9.5 A
AFFX-DapX-M_at 11.9 A
AFFX-DapX-3_at 3.1 A
AFFX-LysX-5_at 0.5 A
AFFX-LysX-M_at 2.8 A
AFFX-LysX-3_at 24.5 P
AFFX-PheX-5_at 1.8 A
AFFX-PheX-M_at 4.3 A
AFFX-PheX-3_at 22.3 A
AFFX-ThrX-5_at 8 A
AFFX-ThrX-M_at 17.1 A

Total number of rows: 15611

Table truncated, full table size 368 Kbytes.




Supplementary file Size Download File type/resource
GSM647638.CEL.gz 2.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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