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Sample GSM6474941 Query DataSets for GSM6474941
Status Public on Aug 22, 2022
Title MKN74 Vehicle 3
Sample type SRA
 
Source name MKN74
Organisms Homo sapiens; Mus musculus
Characteristics cell line: MKN74
cell type: Gastric tumor
treatment: Vehicle
time: Day 3
Treatment protocol When tumor was established, 1mg/kg ERY974 was administered (day 0), and tumor was collected on day 3.
Growth protocol PC10, NCI-H446, MKN74, and MKN45 were expanded in vitro, and inoculated into the humanized mice.
Extracted molecule total RNA
Extraction protocol RNA was harvested from the tumors using RNeasy mini kit (Qiagen). Total RNA was used for RNAseq.
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
 
Description C6AB4ANXX_PR0328_16A06_H1_L008
Data processing The RNAseq reads were mapped to human (GRCh38) and mouse (GRCm38) RefSeq transcripts using bowtie 1.1.2
Reads mapped to the transcripts of both organisms were excluded
The calculation of fragments per kilobase of transcript per million reads mapped (FPKM) using RSEM v1.2.31
Assembly: GRCh38 + CRCm38 with RefSeq annotations
Supplementary files format and content: tab-delimited text files include FPKM values for each Sample
 
Submission date Aug 17, 2022
Last update date Aug 22, 2022
Contact name YUJI SANO
E-mail(s) sanoyuj@chugai-pharm.co.jp
Phone +810467476241
Organization name Chugai Pharmaceutical Co., Ltd
Street address 200
City Kajiwara
State/province Kamakura
ZIP/Postal code 247-8530
Country Japan
 
Platform ID GPL22245
Series (1)
GSE211512 RNA data of baseline and ERY974-treated tumour samples for various xenograft tumors (PC10, NCI-H446, MKN45, and MKN74).
Relations
BioSample SAMN30379762
SRA SRX17126494

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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