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Sample GSM6474937 Query DataSets for GSM6474937
Status Public on Aug 22, 2022
Title NCI-H446 ERY974 2
Sample type SRA
Source name NCI-H446
Organisms Homo sapiens; Mus musculus
Characteristics cell line: NCI-H446
cell type: Lung tumor
treatment: ERY974
time: Day 3
Treatment protocol When tumor was established, 1mg/kg ERY974 was administered (day 0), and tumor was collected on day 3.
Growth protocol PC10, NCI-H446, MKN74, and MKN45 were expanded in vitro, and inoculated into the humanized mice.
Extracted molecule total RNA
Extraction protocol RNA was harvested from the tumors using RNeasy mini kit (Qiagen). Total RNA was used for RNAseq.
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2500
Description C3YKEACXX_PR0158_23A13_H1_L006
Data processing The RNAseq reads were mapped to human (GRCh38) and mouse (GRCm38) RefSeq transcripts using bowtie 1.1.2
Reads mapped to the transcripts of both organisms were excluded
The calculation of fragments per kilobase of transcript per million reads mapped (FPKM) using RSEM v1.2.31
Assembly: GRCh38 + CRCm38 with RefSeq annotations
Supplementary files format and content: tab-delimited text files include FPKM values for each Sample
Submission date Aug 17, 2022
Last update date Aug 22, 2022
Contact name YUJI SANO
Phone +810467476241
Organization name Chugai Pharmaceutical Co., Ltd
Street address 200
City Kajiwara
State/province Kamakura
ZIP/Postal code 247-8530
Country Japan
Platform ID GPL22245
Series (1)
GSE211512 RNA data of baseline and ERY974-treated tumour samples for various xenograft tumors (PC10, NCI-H446, MKN45, and MKN74).
BioSample SAMN30379766
SRA SRX17126490

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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