|
| Status |
Public on Aug 22, 2022 |
| Title |
NCI-H446 ERY974 2 |
| Sample type |
SRA |
| |
|
| Source name |
NCI-H446
|
| Organisms |
Homo sapiens; Mus musculus |
| Characteristics |
cell line: NCI-H446
cell type: Lung tumor
treatment: ERY974
time: Day 3
|
| Treatment protocol |
When tumor was established, 1mg/kg ERY974 was administered (day 0), and tumor was collected on day 3.
|
| Growth protocol |
PC10, NCI-H446, MKN74, and MKN45 were expanded in vitro, and inoculated into the humanized mice.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested from the tumors using RNeasy mini kit (Qiagen). Total RNA was used for RNAseq.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
C3YKEACXX_PR0158_23A13_H1_L006
|
| Data processing |
The RNAseq reads were mapped to human (GRCh38) and mouse (GRCm38) RefSeq transcripts using bowtie 1.1.2
Reads mapped to the transcripts of both organisms were excluded
The calculation of fragments per kilobase of transcript per million reads mapped (FPKM) using RSEM v1.2.31
Assembly: GRCh38 + CRCm38 with RefSeq annotations
Supplementary files format and content: tab-delimited text files include FPKM values for each Sample
|
| |
|
| Submission date |
Aug 17, 2022 |
| Last update date |
Aug 22, 2022 |
| Contact name |
YUJI SANO |
| E-mail(s) |
sanoyuj@chugai-pharm.co.jp
|
| Phone |
+810467476241
|
| Organization name |
Chugai Pharmaceutical Co., Ltd
|
| Street address |
200
|
| City |
Kajiwara |
| State/province |
Kamakura |
| ZIP/Postal code |
247-8530 |
| Country |
Japan |
| |
|
| Platform ID |
GPL22245 |
| Series (1) |
| GSE211512 |
RNA data of baseline and ERY974-treated tumour samples for various xenograft tumors (PC10, NCI-H446, MKN45, and MKN74). |
|
| Relations |
| BioSample |
SAMN30379766 |
| SRA |
SRX17126490 |
