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Sample GSM647017 Query DataSets for GSM647017
Status Public on Dec 01, 2011
Title Xenograft 320, biological rep 3
Sample type RNA
 
Source name GBM Subcutaneous Xenograft
Organism Homo sapiens
Characteristics batch: 10
date of array: 3
line number: 36
line type: Subcutaneous Xenograft
xenograft line: 320
Growth protocol Xenograft lines grown SC or IC until 200 mm^3, cell lines cultured to 70% confluence in either serum free or serum containing media
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Qiagen midi (xenograft), or mini (cell lines) kits. Xenograft tissue pulverized in liquid nitrogen and homogenized using a rotor-stator homogenizer
Label biotin
Label protocol Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 2ug of Total RNA (ExpressionAnalysis Techinical Manual, 2005, Affymetrix).
 
Hybridization protocol Following fragmentation, 10ug of biotinylated cRNA was hybridized for 16hr at 45C onto the Affymetrix Human Genome U133A Plus 2.0 Array. Gene Chips were washed and stained in the Affymetrix Fluidics Station 450 according to standard protocols.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
Data processing data normalized using Bioconductor in R using either RMA or MAS5.0 normalization protocols (RMA provided, see website for MAS5). Batch corrected expression values calculated by factor regression across Affymetrix doping and housekeeping controls
 
Submission date Dec 29, 2010
Last update date Dec 01, 2011
Contact name Jason W Reeves
E-mail(s) jwr11@duke.edu
Organization name Duke University
Lab Nevins, Joseph
Street address 101 Science Dr
City Durham
State/province North Carolina
ZIP/Postal code 27710
Country USA
 
Platform ID GPL571
Series (1)
GSE26344 In vitro models of glioblastoma (GBM)

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
1007_s_at 11.2150
1053_at 8.6966
117_at 5.3120
121_at 7.3233
1255_g_at 3.3568
1294_at 6.3891
1316_at 4.4925
1320_at 4.9687
1405_i_at 3.3874
1431_at 2.8743
1438_at 7.1437
1487_at 7.5261
1494_f_at 4.5693
1598_g_at 6.8833
160020_at 7.9994
1729_at 7.0190
1773_at 5.6199
177_at 4.9734
179_at 8.1622
1861_at 6.2561

Total number of rows: 22277

Table truncated, full table size 391 Kbytes.




Supplementary file Size Download File type/resource
GSM647017.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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