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Sample GSM647015 Query DataSets for GSM647015
Status Public on Dec 01, 2011
Title Xenograft 270, biological rep 4
Sample type RNA
 
Source name GBM Subcutaneous Xenograft
Organism Homo sapiens
Characteristics batch: 10
date of array: 3
line number: 25
line type: Subcutaneous Xenograft
xenograft line: 270
Growth protocol Xenograft lines grown SC or IC until 200 mm^3, cell lines cultured to 70% confluence in either serum free or serum containing media
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Qiagen midi (xenograft), or mini (cell lines) kits. Xenograft tissue pulverized in liquid nitrogen and homogenized using a rotor-stator homogenizer
Label biotin
Label protocol Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 2ug of Total RNA (ExpressionAnalysis Techinical Manual, 2005, Affymetrix).
 
Hybridization protocol Following fragmentation, 10ug of biotinylated cRNA was hybridized for 16hr at 45C onto the Affymetrix Human Genome U133A Plus 2.0 Array. Gene Chips were washed and stained in the Affymetrix Fluidics Station 450 according to standard protocols.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
Data processing data normalized using Bioconductor in R using either RMA or MAS5.0 normalization protocols (RMA provided, see website for MAS5). Batch corrected expression values calculated by factor regression across Affymetrix doping and housekeeping controls
 
Submission date Dec 29, 2010
Last update date Dec 01, 2011
Contact name Jason W Reeves
E-mail(s) jwr11@duke.edu
Organization name Duke University
Lab Nevins, Joseph
Street address 101 Science Dr
City Durham
State/province North Carolina
ZIP/Postal code 27710
Country USA
 
Platform ID GPL571
Series (1)
GSE26344 In vitro models of glioblastoma (GBM)

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
1007_s_at 9.0400
1053_at 8.8351
117_at 4.8108
121_at 7.2700
1255_g_at 3.3355
1294_at 5.8807
1316_at 4.5430
1320_at 5.3590
1405_i_at 3.7199
1431_at 3.1897
1438_at 6.2216
1487_at 7.7152
1494_f_at 4.7750
1598_g_at 7.2230
160020_at 7.7217
1729_at 7.0938
1773_at 5.8090
177_at 4.9024
179_at 8.3553
1861_at 6.0670

Total number of rows: 22277

Table truncated, full table size 391 Kbytes.




Supplementary file Size Download File type/resource
GSM647015.CEL.gz 1.8 Mb (ftp)(http) CEL
Processed data included within Sample table

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