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Sample GSM646953 Query DataSets for GSM646953
Status Public on Dec 01, 2011
Title Xenograft 08-308L, biological rep 2
Sample type RNA
 
Source name GBM Subcutaneous Xenograft
Organism Homo sapiens
Characteristics batch: 5
date of array: 2
line number: 27
line type: Subcutaneous Xenograft
xenograft line: 08-308L
Growth protocol Xenograft lines grown SC or IC until 200 mm^3, cell lines cultured to 70% confluence in either serum free or serum containing media
Extracted molecule total RNA
Extraction protocol Total RNA extracted using Qiagen midi (xenograft), or mini (cell lines) kits. Xenograft tissue pulverized in liquid nitrogen and homogenized using a rotor-stator homogenizer
Label biotin
Label protocol Biotinylated cRNA was prepared according to the standard Affymetrix protocol from 2ug of Total RNA (ExpressionAnalysis Techinical Manual, 2005, Affymetrix).
 
Hybridization protocol Following fragmentation, 10ug of biotinylated cRNA was hybridized for 16hr at 45C onto the Affymetrix Human Genome U133A Plus 2.0 Array. Gene Chips were washed and stained in the Affymetrix Fluidics Station 450 according to standard protocols.
Scan protocol GeneChips were scanned using the Affymetrix GeneChip Scanner 3000 7G
Data processing data normalized using Bioconductor in R using either RMA or MAS5.0 normalization protocols (RMA provided, see website for MAS5). Batch corrected expression values calculated by factor regression across Affymetrix doping and housekeeping controls
 
Submission date Dec 29, 2010
Last update date Dec 01, 2011
Contact name Jason W Reeves
E-mail(s) jwr11@duke.edu
Organization name Duke University
Lab Nevins, Joseph
Street address 101 Science Dr
City Durham
State/province North Carolina
ZIP/Postal code 27710
Country USA
 
Platform ID GPL571
Series (1)
GSE26344 In vitro models of glioblastoma (GBM)

Data table header descriptions
ID_REF
VALUE RMA signal intensity

Data table
ID_REF VALUE
1007_s_at 10.6255
1053_at 8.3668
117_at 6.3138
121_at 6.9755
1255_g_at 3.2744
1294_at 5.7904
1316_at 4.7091
1320_at 4.8824
1405_i_at 3.2542
1431_at 3.4597
1438_at 6.8601
1487_at 7.1473
1494_f_at 4.8184
1598_g_at 7.0938
160020_at 7.2701
1729_at 6.4590
1773_at 6.0009
177_at 4.6110
179_at 8.1673
1861_at 6.6964

Total number of rows: 22277

Table truncated, full table size 391 Kbytes.




Supplementary file Size Download File type/resource
GSM646953.CEL.gz 1.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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