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Sample GSM641605 Query DataSets for GSM641605
Status Public on Dec 29, 2010
Title tumor_9504, expression
Sample type RNA
 
Source name spleen, APL
Organism Mus musculus
Characteristics strain: Bl/6 Taconic
tissue: spleen
disease state: acute promyelocytic leukemia (APL)
Extracted molecule total RNA
Extraction protocol Total cellular RNA was purified using Trizol reagent, quantified using UV spectroscopy (Nanodrop Technologies, Wilmington,DE) and qualitatively assessed using an Experian Bioanalyzer (Hercules,CA). Amplified cDNA was prepared from 20ng of total RNA using the whole transcript WT-Ovation TM RNA Amplification System.
Label biotin
Label protocol Amplified cDNA was biotin labeled using the Encore Biotin Module (NuGen Technologies, San Carlos, CA) according to the manufacturer’s instructions.
 
Hybridization protocol Labeled targets were then hybridized to Mouse Exon 1.0S T arrays (Affymetrix, Santa Clara, CA).
Scan protocol GeneChips were scanned using Affymetrix GeneArray scanner GS 3000.
Data processing Raw data were processed with the RMA algorithm in Affymetrix Expression ConsoleTM version1.1 (Affymetrix Inc., USA).

This analysis included core exons.
Probe-group file: MoEx-1_0-st-v1.r2.pgf
Meta-probeset file: MoEx-1_0-st-v1.r2.dt1.mm9.core.mps
 
Submission date Dec 16, 2010
Last update date Dec 29, 2010
Contact name Nobish Varghese
E-mail(s) nvarghese@path.wustl.edu
Organization name Washington University in St.Louis
Street address 4444 Forest Park Ave.
City StLouis
State/province MO
ZIP/Postal code 63110
Country USA
 
Platform ID GPL6193
Series (2)
GSE26128 Exon array data from mouse APL tumors
GSE26131 Conserved progression mutations revealed by sequencing a mouse acute promyelocytic leukemia genome

Data table header descriptions
ID_REF
VALUE Exon-level RMA signal intensity

Data table
ID_REF VALUE
4304927 45.32517
4304928 2590.302
4304938 223.8152
4304943 1499.069
4304945 412.0019
4304949 219.6804
4304956 125.5452
4304969 63.30476
4304972 157.951
4304978 46.89028
4304981 52.95843
4304986 51.32298
4304997 85.23697
4305011 50.45224
4305016 374.9847
4305019 441.326
4305028 1238.923
4305034 5297.247
4305036 1086.883
4305039 19.62407

Total number of rows: 229581

Table truncated, full table size 3786 Kbytes.




Supplementary file Size Download File type/resource
GSM641605.CEL.gz 24.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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