|
| Status |
Public on Jan 03, 2011 |
| Title |
MEFControl_ChIP-Seq_WCE |
| Sample type |
SRA |
| |
|
| Source name |
Uninduced MEFs
|
| Organism |
Mus musculus |
| Characteristics |
cell division cohort: MEFControl
agent: control
cell type: secondary, inducible MEFs
strain: 129/SvJ x C57/B6 hybrid
chip antibody: none
|
| Treatment protocol |
Either 15% FBS containing medium supplemented with 2 ug/ul Doxycyline or 0.5% FBS containing Serum Starvation medium
|
| Growth protocol |
Standard 10% Fetal Bovine Serum containing growth medium
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Library construction protocol: Single read - Illumina
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina Genome Analyzer II |
| |
|
| Description |
input DNA
|
| Data processing |
Primary sequence data were filtered and aligned to mm8 as described in Mikkelsen et al. Nature 2007, PMID 17603471. The WIG files were similarly created by extending mapped reads 300bp and calculating the overlapping density in 25 bp bins.
|
| |
|
| Submission date |
Dec 16, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Richard Koche |
| Organization name |
Broad Institute of Harvard and MIT
|
| Street address |
185 Cambridge St.
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
|
| Platform ID |
GPL9250 |
| Series (2) |
| GSE26099 |
Widespread targeted chromatin remodeling during the initial phase of somatic cell reprogramming [ChIP-Seq] |
| GSE26100 |
Widespread targeted chromatin remodeling during the initial phase of somatic cell reprogramming |
|
| Relations |
| SRA |
SRX035987 |
| BioSample |
SAMN00188862 |
| Named Annotation |
GSM640770_mm8.MEFcontrol.WCE.wig.gz |
