|
Status |
Public on Jan 03, 2011 |
Title |
MEFdox2Div_rep1 |
Sample type |
RNA |
|
|
Source name |
Doxycycline-induced MEFs
|
Organism |
Mus musculus |
Characteristics |
agent: Doxycycline replicate: 1 cell type: secondary, inducible MEFs strain: 129/SvJ x C57/B6 hybrid cell division cohort: average of two cell divisions
|
Treatment protocol |
Either 15% FBS containing medium supplemented with 2 ug/ul Doxycyline or 0.5% FBS containing Serum Starvation medium
|
Growth protocol |
Standard 10% Fetal Bovine Serum containing growth medium
|
Extracted molecule |
total RNA |
Extraction protocol |
Extraction of total RNA was done using TRIzol (Invitrogen) followed by cleanup using the RNeasy(R) Mini column system (Qiagen).
|
Label |
Biotin
|
Label protocol |
Standard Affymetrix protocol
|
|
|
Hybridization protocol |
Standard Affymetrix protocol
|
Scan protocol |
Standard Affymetrix protocol
|
Description |
Gene expression from 96 hours of doxycycline-induced MEFs with an average of two cell divisions.
|
Data processing |
Preprocessing, normalization, and analysis were all performed using GenePattern (http://www.broadinstitute.org/cancer/software/genepattern/).
|
|
|
Submission date |
Dec 16, 2010 |
Last update date |
Jan 03, 2011 |
Contact name |
Richard Koche |
Organization name |
Broad Institute of Harvard and MIT
|
Street address |
185 Cambridge St.
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02114 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (2) |
GSE26096 |
Widespread targeted chromatin remodeling during the initial phase of somatic cell reprogramming [expression] |
GSE26100 |
Widespread targeted chromatin remodeling during the initial phase of somatic cell reprogramming |
|