|
| Status |
Public on Jan 03, 2011 |
| Title |
MEFdox2Div_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Doxycycline-induced MEFs
|
| Organism |
Mus musculus |
| Characteristics |
agent: Doxycycline
replicate: 1
cell type: secondary, inducible MEFs
strain: 129/SvJ x C57/B6 hybrid
cell division cohort: average of two cell divisions
|
| Treatment protocol |
Either 15% FBS containing medium supplemented with 2 ug/ul Doxycyline or 0.5% FBS containing Serum Starvation medium
|
| Growth protocol |
Standard 10% Fetal Bovine Serum containing growth medium
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was done using TRIzol (Invitrogen) followed by cleanup using the RNeasy(R) Mini column system (Qiagen).
|
| Label |
Biotin
|
| Label protocol |
Standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Standard Affymetrix protocol
|
| Scan protocol |
Standard Affymetrix protocol
|
| Description |
Gene expression from 96 hours of doxycycline-induced MEFs with an average of two cell divisions.
|
| Data processing |
Preprocessing, normalization, and analysis were all performed using GenePattern (http://www.broadinstitute.org/cancer/software/genepattern/).
|
| |
|
| Submission date |
Dec 16, 2010 |
| Last update date |
Jan 03, 2011 |
| Contact name |
Richard Koche |
| Organization name |
Broad Institute of Harvard and MIT
|
| Street address |
185 Cambridge St.
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE26096 |
Widespread targeted chromatin remodeling during the initial phase of somatic cell reprogramming [expression] |
| GSE26100 |
Widespread targeted chromatin remodeling during the initial phase of somatic cell reprogramming |
|
