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Sample GSM635124 Query DataSets for GSM635124
Status Public on Dec 07, 2010
Title DU145 ST3-Hel2A-2-3
Sample type RNA
Source name DU145 treated with ST3-Hel2A-2 for 3hr
Organism Homo sapiens
Characteristics cell line: DU145 prostate cancer cell line
agent: ST3-Hel2A-2
Treatment protocol Cells were treated with ST3-Hel2A-2 or DMSO for 3 hr
Growth protocol DU145 prostate cancer cell line was obtained from American Type Culture Collection. Cells were maintained in RPMI 1640 supplemented with 10% heat-inactivated FBS. Cell cultures were maintained at 37°C under a humidified atmosphere of 5% CO2 in an incubator.
Extracted molecule total RNA
Extraction protocol Extraction of total RNA was performed according to the Qiagen RNeasy manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 5 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133A 2.0 arrays. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using Affymetrix GeneChip Scanner 3000 7G.
Description Gene expression data from DU145 treated with ST3-Hel2A-2
Data processing Matrix table was generated using BRB-ArrayTools Version: 4.1.0 - Beta_3 Release (October 2010) using GC-RMA as normalization method.
Submission date Dec 06, 2010
Last update date Dec 09, 2010
Contact name Olga A Timofeeva
Phone 202-687-8810
Fax 202-687-2221
Organization name Georgetown University Medical Center
Department Oncology
Street address 3900 Reservoir Rd NW
City Washington
State/province DC
ZIP/Postal code 20007
Country USA
Platform ID GPL571
Series (2)
GSE25866 Expression data from DU145 cells treated with ST3-Hel2A-2 STAT3 N-domain inhibitor coupled to analysis of genome-wide STAT3 binding sites
GSE25944 Role of STAT3 in DU145 prostate cancer cell line

Data table header descriptions
VALUE log2, gc-rma signal intensity

Data table
1007_s_at 8.652241707
1053_at 8.678167343
117_at 2.226832151
121_at 2.473470449
1255_g_at 2.20089817
1294_at 2.20089817
1316_at 2.20089817
1320_at 2.278864861
1405_i_at 2.20089817
1431_at 2.20089817
1438_at 2.20089817
1487_at 7.659566879
1494_f_at 2.20089817
1598_g_at 2.288751841
160020_at 2.745419979
1729_at 3.639229059
177_at 2.20089817
1773_at 2.20679307
179_at 2.231555462
1861_at 2.310529232

Total number of rows: 22277

Table truncated, full table size 489 Kbytes.

Supplementary file Size Download File type/resource
GSM635124.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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