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Sample GSM6345650 Query DataSets for GSM6345650
Status Public on Dec 04, 2022
Title HET-E3
Sample type SRA
 
Source name Fetal liver
Organism Mus musculus
Characteristics cell type: Hematopoietic progenitors
genotype: hi-Ddx41+/-
treatment: Infected with empty vector
Treatment protocol Cells were incubated with retroviruses for 2 d and washed and resuspended in PBS with 2% FBS prior to FACS isolation.
Growth protocol Cells were cultured in expansion media containing β-estradiol
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy Micro Kit (74004)
RNA libraries were prepared through Illumina TruSeq Stranded Total RNA (rRNA reduction) protocol
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina NovaSeq 6000
 
Description mutant
Data processing Use RSEM to generate gene expression from fastq data
Supplementary files format and content: results: txt file containing TPM and FPKM level
 
Submission date Jul 18, 2022
Last update date Dec 04, 2022
Contact name Siqi Shen
E-mail(s) sshen82@wisc.edu
Organization name University of Wisconsin - Madison
Street address 425 Henry Mall
City MADISON
State/province WI
ZIP/Postal code 53705
Country USA
 
Platform ID GPL24247
Series (1)
GSE208400 Discriminating activities of DEAD-Box Helicase 41 from myeloid malignancy-associated germline variants by genetic rescue
Relations
BioSample SAMN29798604
SRA SRX16319427

Supplementary file Size Download File type/resource
GSM6345650_HET-A3_S70.genes.results.gz 1.8 Mb (ftp)(http) RESULTS
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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