|
| Status |
Public on Dec 04, 2022 |
| Title |
HET-E2 |
| Sample type |
SRA |
| |
|
| Source name |
Fetal liver
|
| Organism |
Mus musculus |
| Characteristics |
cell type: Hematopoietic progenitors
genotype: hi-Ddx41+/-
treatment: Infected with empty vector
|
| Treatment protocol |
Cells were incubated with retroviruses for 2 d and washed and resuspended in PBS with 2% FBS prior to FACS isolation.
|
| Growth protocol |
Cells were cultured in expansion media containing β-estradiol
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Qiagen RNeasy Micro Kit (74004)
RNA libraries were prepared through Illumina TruSeq Stranded Total RNA (rRNA reduction) protocol
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
mutant
|
| Data processing |
Use RSEM to generate gene expression from fastq data
Supplementary files format and content: results: txt file containing TPM and FPKM level
|
| |
|
| Submission date |
Jul 18, 2022 |
| Last update date |
Dec 04, 2022 |
| Contact name |
Siqi Shen |
| E-mail(s) |
sshen82@wisc.edu
|
| Organization name |
University of Wisconsin - Madison
|
| Street address |
425 Henry Mall
|
| City |
MADISON |
| State/province |
WI |
| ZIP/Postal code |
53705 |
| Country |
USA |
| |
|
| Platform ID |
GPL24247 |
| Series (1) |
| GSE208400 |
Discriminating activities of DEAD-Box Helicase 41 from myeloid malignancy-associated germline variants by genetic rescue |
|
| Relations |
| BioSample |
SAMN29798605 |
| SRA |
SRX16319426 |
