|
| Status |
Public on Dec 01, 2011 |
| Title |
Col-0_grown_at 30C |
| Sample type |
SRA |
| |
|
| Source name |
Col-0_grown_at 30℃
|
| Organism |
Arabidopsis thaliana |
| Characteristics |
ecotype: Col-0
ecotype: wildtype
cultivation temperature: 30℃
age: 4-week-old
tissue: seedlings
|
| Treatment protocol |
Total RNA was isolated from 4-week-old 22ºC or 30ºC-grown plants.
|
| Growth protocol |
Arabidopsis Col-0 and NRG1 transgenic plants were grow at 22°C or 30°C for 4 weeks.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Small RNAs were prepared from Arabidopsis seedings, 16-28 nt size fractionation was separated from total RNA and ligated to a 5' and a 3' adaptor sequentially, and then RT-PCR amplified. PCR products were provided for sequencing.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina Genome Analyzer |
| |
|
| Description |
Solexa gene sequencing
|
| Data processing |
The 18-30nt tags from Solexa sequencing will go through the primary analysis first, which includes getting rid of the 5' adaptor, low quality tags and summarise the length distribution of the clean tags.
|
| |
|
| Submission date |
Dec 01, 2010 |
| Last update date |
May 15, 2019 |
| Contact name |
Li Qun |
| E-mail(s) |
qli@sibs.ac.cn
|
| Phone |
+86 21 54924122
|
| Organization name |
Institute of plant physiology & ecology, SIBS, CAS
|
| Department |
National Key Laboratory of Plant Molecular Genetics
|
| Street address |
300 Fenglin Road
|
| City |
Shanghai |
| State/province |
Shanghai |
| ZIP/Postal code |
200032 |
| Country |
China |
| |
|
| Platform ID |
GPL9062 |
| Series (1) |
| GSE25738 |
Examination of smallRNA in wildtype and transgenic Arabidopsis grown under different temperature |
|
| Relations |
| SRA |
SRX032393 |
| BioSample |
SAMN00139221 |
