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Sample GSM6311175 Query DataSets for GSM6311175
Status Public on Jul 11, 2022
Title OE1
Sample type SRA
Source name mouse embryonic fibroblasts
Organism Mus musculus
Characteristics cell type: embryonic fibroblasts
condition: mouse embryonic fibroblasts induced to overexpress Bcl11b
strain: C57BL/6
Treatment protocol MEF are infected with Bcl11b shRNA targetting particles or overexpressing plasmids and RNA were collected after 3 days
Growth protocol MEF were generated from E13.5 reprogrammable mice embryos and cultivated in DMEM medium supplemented with 10% foetal bovine serum (FBS), 100U/ml penicillin / streptomycin (PS), 1mM sodium pyruvate, 2mM L-glutamine, 0.1mM Non Essential Amino Acids (NEAA) and 0.1mM b-mercaptoethanol.
Extracted molecule total RNA
Extraction protocol RNAs were extracted with Nucleospin RNA kit from Macherey Nagel.
RNA libraries were prepared for sequencing using standard Illumina protocols
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 4000
Data processing Sequenced reads were mapped to mm8 (release M19) whole genome and reads per gene were counted using STAR (ver. 2.5b).
Read counts were analyzed with R DeSeq2 package.
Supplementary files format and content: raw counts, normalized counts, csv file including FC, p-value and adjusted p-value
Submission date Jul 08, 2022
Last update date Jul 11, 2022
Contact name Fabrice Lavial
Organization name CRCL
Street address 28 rue Laennec
City Lyon
ZIP/Postal code 69008
Country France
Platform ID GPL21103
Series (2)
GSE137050 The comparative roadmaps of reprogramming and transformation unveiled that cellular plasticity is broadly controlled by Bcl11b and Atoh8
GSE207732 Identification of genes regulated by Bcl11b
BioSample SAMN29598067
SRA SRX16085349

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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