|Public on Jul 11, 2022
|mouse embryonic fibroblasts
|cell type: embryonic fibroblasts
condition: mouse embryonic fibroblasts induced to overexpress Bcl11b
|MEF are infected with Bcl11b shRNA targetting particles or overexpressing plasmids and RNA were collected after 3 days
|MEF were generated from E13.5 reprogrammable mice embryos and cultivated in DMEM medium supplemented with 10% foetal bovine serum (FBS), 100U/ml penicillin / streptomycin (PS), 1mM sodium pyruvate, 2mM L-glutamine, 0.1mM Non Essential Amino Acids (NEAA) and 0.1mM b-mercaptoethanol.
|RNAs were extracted with Nucleospin RNA kit from Macherey Nagel.
RNA libraries were prepared for sequencing using standard Illumina protocols
|Illumina HiSeq 4000
|Sequenced reads were mapped to mm8 (release M19) whole genome and reads per gene were counted using STAR (ver. 2.5b).
Read counts were analyzed with R DeSeq2 package.
Supplementary files format and content: raw counts, normalized counts, csv file including FC, p-value and adjusted p-value
|Jul 08, 2022
|Last update date
|Jul 11, 2022
|28 rue Laennec
|The comparative roadmaps of reprogramming and transformation unveiled that cellular plasticity is broadly controlled by Bcl11b and Atoh8
|Identification of genes regulated by Bcl11b