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Sample GSM63081 Query DataSets for GSM63081
Status Public on Mar 16, 2006
Title HVSMC_ Thapsigargin_treated_rep2
Sample type RNA
 
Source name human cerebral artery
Organism Homo sapiens
Characteristics Gender: Male
Age: 45 years
Tissue: human cerebral artery
Biomaterial provider Human cerebral vascular smooth muscle cells (hcVSMCs) (passages 2-4) were obtained from human cerebral artery explants (IRB# CHRMS 01-195; informed consent) and maintained in SMGM2 (Clontech, Palo Alto, CA
Treatment protocol Early passage hcVSMCs (passage 2-4) were obtained from human cerebral artery explants. Briefly, 2 mm slices of human pial artery (IRB# CHRMS 01-195; informed consent) were applied to scored 60 mm culture dishes, cultured in SMGM2 media (Cambrex Bio Science, Hopkinton, MA), and passaged with trypsin/EDTA. For hypoxia exposure, cells were incubated at 2% O2 by N2 injection into a humidified CO2 incubator (Forma, Marietta, OH). NOC-18, Cu/Zn superoxide dismutase (SOD), catalase, xanthine, and xanthine oxidase were obtained from Calbiochem (San Diego, CA). S-nitroso-L-glutathione (GSNO) was obtained from Alexis
Growth protocol hcVSMCs and rat VSMCs (passages 2-4) were grown to approximately 60% confluence and serum starved in DMEM containing 0.1% FBS 24-48 hr prior to treatment
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from treated hc VSMCs using TriZol reagent and chloroform. RNA was precipitated using isoproponol and glycoblue, washed with 75% ethanol, dissolved in RNAse free water and quantified using the NanoDrop® spectrophotometer.
Label biotin-labeled
Label protocol Double-stranded cDNA was then synthesized followed by an in vitro transcription reaction and fragmentation reaction to produce biotin-labeled cRNA fragments that were hybridized to the probe array at 45°C for 16 hr
 
Hybridization protocol biotin-labeled cRNA fragments that were hybridized to the probe array at 45°C for 16 hr
Scan protocol The probe arrays were scanned (Hewlett-Packard GeneArray Scanner, Agilent Technologies, Inc.) to quantify the fluorescence intensity associated with each oligonucleotide probe.
Description each of three experiments cell cultures were split three ways; one of the resulting samples was left untreated (C), another was treated with thapsigargin (TG), and the third was treated with elevated K+ (K)
Data processing RMA
 
Submission date Jul 06, 2005
Last update date Mar 16, 2006
Contact name Anjanette D Watson
E-mail(s) Anjanette.Watson@uvm.edu
Phone 8026568612
Organization name University of Vermont
Department Bioinformatics Core
Street address 120A Marsh Life Science
City Burlington
State/province VT
ZIP/Postal code 05405
Country USA
 
Platform ID GPL96
Series (1)
GSE2883 Ca2+-dependent Transcription Patterns in Human Cerebrovascular Smooth Muscle

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
1007_s_at 10.1692211222140
1053_at 6.48144906605807
117_at 7.2960574102182
121_at 10.1333750978408
1255_g_at 5.37201787560866
1294_at 8.48587464801894
1316_at 6.35001455087296
1320_at 7.33811319152993
1405_i_at 5.04617957726864
1431_at 5.16619028499255
1438_at 7.89353498415984
1487_at 8.52044156759004
1494_f_at 8.02961068026143
1598_g_at 9.96478649183517
160020_at 9.06549353694647
1729_at 8.76081569055391
1773_at 7.60974416227412
177_at 7.44328560099469
179_at 10.3110271988452
1861_at 7.9640478045859

Total number of rows: 22279

Table truncated, full table size 604 Kbytes.




Supplementary data files not provided

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