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Sample GSM62957 Query DataSets for GSM62957
Status Public on Jul 05, 2006
Title left ventricular- 4 months old- lean 2
Sample type RNA
 
Source name 4 months old SHHF lean rat, left ventricular
Organism Rattus norvegicus
Characteristics Strain: SHHF, Gender: Male,
Age: 4 months, Tissue: left ventricular, Other: hypertension, dilated cardiomyopathy starting from 14 months old
Biomaterial provider Charles River laboratory
Treatment protocol None
Growth protocol Animals were lodged in the Zootechnic service of the Louis Bugnard Institute. A veterinary oversees animal health and management. Temperature : 22 +/- 1 degrees. Cycles nights/days : 12 hours. Food : Teklad Global 16% Protein Rodent Diet (Harlan)
Extracted molecule total RNA
Extraction protocol General anesthesia by Isoflurane 4%. Heart extraction. Isolation of total RNA from left ventricular by : TRIzol (Invitrogen), homogeneisor Ika Ultra-Thurax T 25 (Janke and Kunkel), DNAfree (Ambion). Quality and quantity were determined by capillary electrophoresis (Agilent).
Label 33P
Label protocol Reverse Transcription of 3 µg total RNA from each left ventricular with Superscript enzyme (Invitrogen). Purification of cDNA labelled with 33P with NucleoSpin Extract (Macherey-Nagel)
 
Hybridization protocol Using one nylon membrane per animal for the hybridization (kit Array-Advantage GF, Ambion)
Scan protocol Exposition of each membrane during 24 hours (Storage Phosphor Screen, Molecular Dynamics). Revelation with Scan Typhoon 9400 (resolution at 50 µm per pixel). Analysis of images by Image Quant 5.1
Description Lean rats can be homozygous or heterozygous for the leptin receptor gene (+/+ or cp/+, cp means corpulent allele and is a recessive autosomic one, + means wild type)
Data processing background subtraction, log transformation, normalization by total mean, exponential (back from log), scaling by a factor 10000
 
Submission date Jul 04, 2005
Last update date Oct 28, 2005
Contact name Philippe V Rouet
E-mail(s) Philippe.rouet@inserm.fr
Phone 00 33 5 31 22 41 19
Organization name INSERM U1048
Department Cardiology
Lab Team 7
Street address 1 Ave Jean Poulhes
City Toulouse
State/province BP84225
ZIP/Postal code 31432
Country France
 
Platform ID GPL2580
Series (1)
GSE2876 Obesity impact on the left ventricular transcriptome of 4 and 10 months old SHHF rats

Data table header descriptions
ID_REF
SIGNAL_RAW Raw Intensity
BKD_RAW Raw Background
VALUE Normalized Intensity

Data table
ID_REF SIGNAL_RAW BKD_RAW VALUE
240_240 3641.15 2772.17 13055
240_239 6552.04 2186.67 65582.3
240_238 2655.25 1945.67 10660.2
240_237 7090.83 1683.33 81238.6
240_236 1992.53 1482.33 7664.9
240_235 9910.32 1362.5 128417
240_234 1648.43 947.33 10532.8
240_233 1624.88 887.17 11082.9
240_232 5005.82 878.83 62001.1
240_231 1313.07 770 8158.71
240_230 1193.41 719.17 7124.66
240_229 1474.07 647.5 12417.8
240_228 1441.31 664.33 11672.8
240_227 1514.97 703.67 12188.4
240_226 982.67 532.33 6765.6
240_225 1180.67 447.83 11009.7
240_224 976.51 499 7173.78
240_223 2124.75 588.5 23079.6
240_222 938.19 563.17 5634.04
240_221 1051.44 457.33 8925.5

Total number of rows: 57600

Table truncated, full table size 1622 Kbytes.




Supplementary file Size Download File type/resource
GSM62957.xls.gz 3.7 Mb (ftp)(http) XLS

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