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| Status |
Public on Nov 10, 2023 |
| Title |
ChIP DNA, 2% Input rep 1 |
| Sample type |
SRA |
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| Source name |
Spleen
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| Organism |
Mus musculus |
| Characteristics |
tissue: Spleen
antibody: none
cell type: Th2
genotype: C57BL/6
treatment: Activated for five days in vitro with irradiated APCs, aCD3, aCD28, aIFNg, aIL-12, and IL-4.
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| Growth protocol |
Activated for five days in vitro with irradiated APCs, aCD3, aCD28, aIFNg, aIL-12, and IL-4.
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| Extracted molecule |
genomic DNA |
| Extraction protocol |
Cells were fixed with 1% formaldehyde for 10 minutes at room temperature. After quenching and cell lysis, chromatin was digested and immunoprecipitated as indiciated in the Cell Signaling Technology Simple ChIP protocol.
Libraries were prepared as indicated in the Cell Signaling Technology Simple ChIP library single-index library construction kit.
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| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
NextSeq 2000 |
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| Data processing |
ChIP-seq reads were aligned with Bowtie2 to the mm10 genome
Reads were sorted and indexed with samtools
Count per million bigwig traces were generated with Deeptools
Assembly: mm10
Supplementary files format and content: bigwig
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| Submission date |
Jul 05, 2022 |
| Last update date |
Nov 10, 2023 |
| Contact name |
Remy Bosselut |
| E-mail(s) |
bosselur@helix.nih.gov
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| Phone |
240-760-6866
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| Organization name |
National Cancer Institute, National Institute of Health
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| Department |
Laboratory of Immune Cell Biology
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| Lab |
Bosselut
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| Street address |
9000 Rockville Pike, Building 37
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platform ID |
GPL30172 |
| Series (2) |
| GSE207469 |
Zfp281 and Zfp148 control CD4+ T cell thymic development and Th2 functions. (ChIP-seq) |
| GSE207572 |
Zfp281 and Zfp148 control CD4+ T cell thymic development and Th2 functions |
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| Relations |
| BioSample |
SAMN29500353 |
| SRA |
SRX16019901 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSM6288249_Th2_Input_Rep1_cpm.bw |
257.0 Mb |
(ftp)(http) |
BW |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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