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Sample GSM627583 Query DataSets for GSM627583
Status Public on Nov 23, 2010
Title CD16 Control
Sample type RNA
Source name unstimulated CD14loCD16+ monocytes from peripheral human blood
Organism Homo sapiens
Characteristics cell type: CD14loCD16+ monocytes isolated from peripheral human blood by CD16 microbeads
Biomaterial provider Lab D3, University of Texas Health Scienece Center at Tyler
Treatment protocol untreated
Growth protocol CD14loCD16+ monocytes were isolated from human peripheral blood using magnetic beads for CD16. 1.5 million cells were cultured in 1 ml of complete RPMI medium with 10% human serum.
Extracted molecule total RNA
Extraction protocol After 48 hours stimulated or control CD14loCD16+ monocytes were harvested and RNA was isolated using Trizol (Invitrogen) acc. to manufacturer's protocol.
Label Cy5
Label protocol See for updated protocols
Hybridization protocol See for updated protocols
Scan protocol See for updated protocols
Data processing See for updated protocols and additional information
Submission date Nov 22, 2010
Last update date Nov 22, 2010
Contact name Rohan Dhiman
Phone 903-8775385
Fax 903-8777989
Organization name University of Texas Health Science Center at Tyler
Department Centre for Pulmonary and Infectious Disease Control
Lab D3
Street address 11937 US Hwy 271
City Tyler
State/province Texas
ZIP/Postal code 75708
Country USA
Platform ID GPL6254
Series (1)
GSE25505 Gene expression analysis of gamma-M.tuberculosis-stimulated CD14hiCD16- and CD14loCD16+ monocytes

Data table header descriptions
Control1 CD16 Control value 1
Control2 CD16 Control value 2
VALUE Normalized value (average)

Data table
ID_REF Control1 Control2 VALUE
PH_hs_0000003 802.625 749 775.8125
PH_hs_0000006 2000.25 2031.25 2015.75
PH_hs_0000007 1820.5 2106.75 1963.625
PH_hs_0000008 430.625 570.25 500.4375
PH_hs_0000009 1489.25 1307 1398.125
PH_hs_0000010 6728.5 7223.75 6976.125
PH_hs_0000011 282.75 146.75 214.75
PH_hs_0000012 5109.25 4625.25 4867.25
PH_hs_0000013 691.75 644.625 668.1875
PH_hs_0000014 336 294.875 315.4375
PH_hs_0000018 250 329.125 289.5625
PH_hs_0000019 248.25 205.5 226.875
PH_hs_0000020 6548.75 5891.25 6220

Total number of rows: 30968

Table truncated, full table size 691 Kbytes.

Supplementary file Size Download File type/resource
GSM627583.txt.gz 229.1 Kb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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