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Sample GSM627583 Query DataSets for GSM627583
Status Public on Nov 23, 2010
Title CD16 Control
Sample type RNA
 
Source name unstimulated CD14loCD16+ monocytes from peripheral human blood
Organism Homo sapiens
Characteristics cell type: CD14loCD16+ monocytes isolated from peripheral human blood by CD16 microbeads
Biomaterial provider Lab D3, University of Texas Health Scienece Center at Tyler
Treatment protocol untreated
Growth protocol CD14loCD16+ monocytes were isolated from human peripheral blood using magnetic beads for CD16. 1.5 million cells were cultured in 1 ml of complete RPMI medium with 10% human serum.
Extracted molecule total RNA
Extraction protocol After 48 hours stimulated or control CD14loCD16+ monocytes were harvested and RNA was isolated using Trizol (Invitrogen) acc. to manufacturer's protocol.
Label Cy5
Label protocol See http://www.phalanxbiotech.com/ for updated protocols
 
Hybridization protocol See http://www.phalanxbiotech.com/ for updated protocols
Scan protocol See http://www.phalanxbiotech.com/ for updated protocols
Data processing See http://www.phalanxbiotech.com/ for updated protocols and additional information
 
Submission date Nov 22, 2010
Last update date Nov 22, 2010
Contact name Rohan Dhiman
E-mail(s) rohandhiman79@yahoo.com
Phone 903-8775385
Fax 903-8777989
Organization name University of Texas Health Science Center at Tyler
Department Centre for Pulmonary and Infectious Disease Control
Lab D3
Street address 11937 US Hwy 271
City Tyler
State/province Texas
ZIP/Postal code 75708
Country USA
 
Platform ID GPL6254
Series (1)
GSE25505 Gene expression analysis of gamma-M.tuberculosis-stimulated CD14hiCD16- and CD14loCD16+ monocytes

Data table header descriptions
ID_REF
Control1 CD16 Control value 1
Control2 CD16 Control value 2
VALUE Normalized value (average)

Data table
ID_REF Control1 Control2 VALUE
PH_hs_0000002
PH_hs_0000003 802.625 749 775.8125
PH_hs_0000004
PH_hs_0000005
PH_hs_0000006 2000.25 2031.25 2015.75
PH_hs_0000007 1820.5 2106.75 1963.625
PH_hs_0000008 430.625 570.25 500.4375
PH_hs_0000009 1489.25 1307 1398.125
PH_hs_0000010 6728.5 7223.75 6976.125
PH_hs_0000011 282.75 146.75 214.75
PH_hs_0000012 5109.25 4625.25 4867.25
PH_hs_0000013 691.75 644.625 668.1875
PH_hs_0000014 336 294.875 315.4375
PH_hs_0000015
PH_hs_0000016
PH_hs_0000017
PH_hs_0000018 250 329.125 289.5625
PH_hs_0000019 248.25 205.5 226.875
PH_hs_0000020 6548.75 5891.25 6220
PH_hs_0000021

Total number of rows: 30968

Table truncated, full table size 691 Kbytes.




Supplementary file Size Download File type/resource
GSM627583.txt.gz 229.1 Kb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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