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Sample GSM626353 Query DataSets for GSM626353
Status Public on Nov 30, 2011
Title Feproexpress AP 14
Sample type RNA
 
Source name Liver
Organism Sus scrofa
Characteristics breed: German Landrace
tissue: liver
ontogenetic stage: 94 dpc
gestation diet: offspring of German Landrace gilts fed a gestational adequate protein diet
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using TRI Reagent (Sigma, Taufkirchen, Germany) according to the manufacturer’s protocol. After DNaseI treatment the RNA was cleaned up using the RNeasy Kit (Qiagen, Hilden, Germany).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.5 ug total RNA (Expression Analysis Technical Manual, 2009, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 42C on Affymetrix GeneChip Porcine Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol Scanning using the GeneChip scanner 3000 were performed according to Affymetrix protocols
Description Gene expression data from liver sample of offspring
Data processing Data were analysed with Affymetrix GCOS 1.1.1 software using global scaling to a target signal of 500 and BioConductor.
 
Submission date Nov 18, 2010
Last update date Nov 30, 2011
Contact name Klaus Wimmers
E-mail(s) s.wimmers@fbn-dummerstorf.de
Phone 0049 3820868700
Organization name Leibniz Institute for Farm Animal Biology (FBN)
Street address Wilhelm-Stahl-Allee 2
City Dummerstorf
ZIP/Postal code 18196
Country Germany
 
Platform ID GPL3533
Series (2)
GSE25482 Fetal programming of hepatic transcriptome in response to gestational dietary protein levels in the pig (AP data set)
GSE25484 Fetal programming of hepatic transcriptome in response to gestational dietary protein levels in the pig

Data table header descriptions
ID_REF
VALUE MAS5.0 signal intensity
ABS_CALL
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 846.4203 P 0.000445901
AFFX-BioB-M_at 1060.783 P 4.43E-05
AFFX-BioB-3_at 793.7844 P 4.43E-05
AFFX-BioC-5_at 2531.63 P 5.17E-05
AFFX-BioC-3_at 3472.481 P 4.43E-05
AFFX-BioDn-5_at 5993.154 P 4.43E-05
AFFX-BioDn-3_at 12424.57 P 0.000258358
AFFX-CreX-5_at 40047.69 P 5.17E-05
AFFX-CreX-3_at 42584.84 P 4.43E-05
AFFX-DapX-5_at 3493.582 P 4.43E-05
AFFX-DapX-M_at 5415.46 P 0.000126798
AFFX-DapX-3_at 6585.975 P 4.43E-05
AFFX-LysX-5_at 390.7673 P 4.42E-05
AFFX-LysX-M_at 386.1523 P 0.002866996
AFFX-LysX-3_at 690.0876 P 4.43E-05
AFFX-PheX-5_at 488.7944 P 6.02E-05
AFFX-PheX-M_at 534.7877 P 0.001101968
AFFX-PheX-3_at 518.8434 P 0.00141043
AFFX-ThrX-5_at 669.5691 P 9.45E-05
AFFX-ThrX-M_at 912.6464 P 4.43E-05

Total number of rows: 24123

Table truncated, full table size 912 Kbytes.




Supplementary file Size Download File type/resource
GSM626353.CEL.gz 2.0 Mb (ftp)(http) CEL
Processed data included within Sample table

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