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Sample GSM626350 Query DataSets for GSM626350
Status Public on Nov 30, 2011
Title Feproexpress AP 11
Sample type RNA
Source name Liver
Organism Sus scrofa
Characteristics breed: German Landrace
tissue: liver
ontogenetic stage: 94 dpc
gestation diet: offspring of German Landrace gilts fed a gestational adequate protein diet
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using TRI Reagent (Sigma, Taufkirchen, Germany) according to the manufacturer’s protocol. After DNaseI treatment the RNA was cleaned up using the RNeasy Kit (Qiagen, Hilden, Germany).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.5 ug total RNA (Expression Analysis Technical Manual, 2009, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 42C on Affymetrix GeneChip Porcine Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol Scanning using the GeneChip scanner 3000 were performed according to Affymetrix protocols
Description Gene expression data from liver sample of offspring
Data processing Data were analysed with Affymetrix GCOS 1.1.1 software using global scaling to a target signal of 500 and BioConductor.
Submission date Nov 18, 2010
Last update date Nov 30, 2011
Contact name Klaus Wimmers
Phone 0049 3820868700
Organization name Leibniz Institute for Farm Animal Biology (FBN)
Street address Wilhelm-Stahl-Allee 2
City Dummerstorf
ZIP/Postal code 18196
Country Germany
Platform ID GPL3533
Series (2)
GSE25482 Fetal programming of hepatic transcriptome in response to gestational dietary protein levels in the pig (AP data set)
GSE25484 Fetal programming of hepatic transcriptome in response to gestational dietary protein levels in the pig

Data table header descriptions
VALUE MAS5.0 signal intensity

Data table
AFFX-BioB-5_at 1028.564 P 0.000509415
AFFX-BioB-M_at 1640.902 P 4.43E-05
AFFX-BioB-3_at 1087.832 P 4.43E-05
AFFX-BioC-5_at 3580.964 P 6.02E-05
AFFX-BioC-3_at 4570.782 P 4.43E-05
AFFX-BioDn-5_at 7932.994 P 4.43E-05
AFFX-BioDn-3_at 15523.86 P 0.000169227
AFFX-CreX-5_at 52303.19 P 5.17E-05
AFFX-CreX-3_at 56496.11 P 4.43E-05
AFFX-DapX-5_at 2791.802 P 5.17E-05
AFFX-DapX-M_at 4460.748 P 9.45E-05
AFFX-DapX-3_at 5270.959 P 4.43E-05
AFFX-LysX-5_at 321.4118 P 9.45E-05
AFFX-LysX-M_at 314.9623 P 0.002555524
AFFX-LysX-3_at 441.1981 P 0.000146581
AFFX-PheX-5_at 376.2162 P 6.01E-05
AFFX-PheX-M_at 382.8088 P 0.000581214
AFFX-PheX-3_at 489.5982 P 0.000972149
AFFX-ThrX-5_at 715.4308 P 0.000126798
AFFX-ThrX-M_at 836.0914 P 4.42E-05

Total number of rows: 24123

Table truncated, full table size 912 Kbytes.

Supplementary file Size Download File type/resource
GSM626350.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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