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Sample GSM626342 Query DataSets for GSM626342
Status Public on Nov 30, 2011
Title Feproexpress AP 03
Sample type RNA
Source name Liver
Organism Sus scrofa
Characteristics breed: German Landrace
tissue: liver
ontogenetic stage: 94 dpc
gestation diet: offspring of German Landrace gilts fed a gestational adequate protein diet
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using TRI Reagent (Sigma, Taufkirchen, Germany) according to the manufacturer’s protocol. After DNaseI treatment the RNA was cleaned up using the RNeasy Kit (Qiagen, Hilden, Germany).
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 0.5 ug total RNA (Expression Analysis Technical Manual, 2009, Affymetrix).
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 42C on Affymetrix GeneChip Porcine Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol Scanning using the GeneChip scanner 3000 were performed according to Affymetrix protocols
Description Gene expression data from liver sample of offspring
Data processing Data were analysed with Affymetrix GCOS 1.1.1 software using global scaling to a target signal of 500 and BioConductor.
Submission date Nov 18, 2010
Last update date Nov 30, 2011
Contact name Klaus Wimmers
Phone 0049 3820868700
Organization name Leibniz Institute for Farm Animal Biology (FBN)
Street address Wilhelm-Stahl-Allee 2
City Dummerstorf
ZIP/Postal code 18196
Country Germany
Platform ID GPL3533
Series (2)
GSE25482 Fetal programming of hepatic transcriptome in response to gestational dietary protein levels in the pig (AP data set)
GSE25484 Fetal programming of hepatic transcriptome in response to gestational dietary protein levels in the pig

Data table header descriptions
VALUE MAS5.0 signal intensity

Data table
AFFX-BioB-5_at 946.1379 P 0.000389797
AFFX-BioB-M_at 1222.356 P 4.43E-05
AFFX-BioB-3_at 915.8943 P 4.43E-05
AFFX-BioC-5_at 3061.018 P 6.02E-05
AFFX-BioC-3_at 3940.506 P 4.43E-05
AFFX-BioDn-5_at 6953.212 P 4.43E-05
AFFX-BioDn-3_at 14319.2 P 0.000224668
AFFX-CreX-5_at 44758.08 P 5.17E-05
AFFX-CreX-3_at 47574.5 P 4.43E-05
AFFX-DapX-5_at 2402.218 P 4.43E-05
AFFX-DapX-M_at 3869.935 P 0.000126798
AFFX-DapX-3_at 4200.531 P 4.43E-05
AFFX-LysX-5_at 258.0199 P 6.02E-05
AFFX-LysX-M_at 288.148 P 0.008440466
AFFX-LysX-3_at 400.5577 P 4.43E-05
AFFX-PheX-5_at 308.3943 P 7.01E-05
AFFX-PheX-M_at 462.6427 P 0.000389797
AFFX-PheX-3_at 416.6074 P 0.003594584
AFFX-ThrX-5_at 664.6234 P 0.000258358
AFFX-ThrX-M_at 669.7797 P 4.43E-05

Total number of rows: 24123

Table truncated, full table size 913 Kbytes.

Supplementary file Size Download File type/resource
GSM626342.CEL.gz 1.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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