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Sample GSM6256709 Query DataSets for GSM6256709
Status Public on Nov 10, 2023
Title LCZ11 Zfp148/281 dKO ImCD8 thymocyte
Sample type SRA
 
Source name Thymus
Organism Mus musculus
Characteristics tissue: Thymus
cell type: ImCD8
genotype: Zfp148 fl/fl; Zfp281 fl/fl; Cd4-Cre negative
flow markers: CD44low, CD8+ CD4-, TCRb+, CD69+
Extracted molecule total RNA
Extraction protocol RNA was isolated using the RNeasy Plus Micro kit (Qiagen)
Clontech SMARTer Low Input mRNA following the manufacturer's protocol
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 4000
 
Data processing Filtering with Cutadapt 1.18
Alignment ot mm10 with STAR 2.6.1
Read counting with HTseq
Assembly: mm10
Supplementary files format and content: Htseq count table
 
Submission date Jun 21, 2022
Last update date Nov 10, 2023
Contact name Remy Bosselut
E-mail(s) bosselur@helix.nih.gov
Phone 240-760-6866
Organization name National Cancer Institute, National Institute of Health
Department Laboratory of Immune Cell Biology
Lab Bosselut
Street address 9000 Rockville Pike, Building 37
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
 
Platform ID GPL21103
Series (2)
GSE206542 The transcription factor Zfp281 serves redundantly with Zfp148 to support CD4+ T cell development and functions [Population RNA-seq]
GSE207572 Zfp281 and Zfp148 control CD4+ T cell thymic development and Th2 functions
Relations
BioSample SAMN29535817
SRA SRX16041385

Supplementary file Size Download File type/resource
GSM6256709_LCZ11_htseq.tab.gz 181.2 Kb (ftp)(http) TAB
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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