|
Status |
Public on Nov 10, 2023 |
Title |
LCZ11 Zfp148/281 dKO ImCD8 thymocyte |
Sample type |
SRA |
|
|
Source name |
Thymus
|
Organism |
Mus musculus |
Characteristics |
tissue: Thymus cell type: ImCD8 genotype: Zfp148 fl/fl; Zfp281 fl/fl; Cd4-Cre negative flow markers: CD44low, CD8+ CD4-, TCRb+, CD69+
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was isolated using the RNeasy Plus Micro kit (Qiagen) Clontech SMARTer Low Input mRNA following the manufacturer's protocol
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 4000 |
|
|
Data processing |
Filtering with Cutadapt 1.18 Alignment ot mm10 with STAR 2.6.1 Read counting with HTseq Assembly: mm10 Supplementary files format and content: Htseq count table
|
|
|
Submission date |
Jun 21, 2022 |
Last update date |
Nov 10, 2023 |
Contact name |
Remy Bosselut |
E-mail(s) |
bosselur@helix.nih.gov
|
Phone |
240-760-6866
|
Organization name |
National Cancer Institute, National Institute of Health
|
Department |
Laboratory of Immune Cell Biology
|
Lab |
Bosselut
|
Street address |
9000 Rockville Pike, Building 37
|
City |
Bethesda |
State/province |
MD |
ZIP/Postal code |
20892 |
Country |
USA |
|
|
Platform ID |
GPL21103 |
Series (2) |
GSE206542 |
The transcription factor Zfp281 serves redundantly with Zfp148 to support CD4+ T cell development and functions [Population RNA-seq] |
GSE207572 |
Zfp281 and Zfp148 control CD4+ T cell thymic development and Th2 functions |
|
Relations |
BioSample |
SAMN29535817 |
SRA |
SRX16041385 |