|
| Status |
Public on Nov 10, 2023 |
| Title |
dKO, thymocyte, rep2 |
| Sample type |
SRA |
| |
|
| Source name |
Thymus
|
| Organism |
Mus musculus |
| Characteristics |
tissue: Thymus
replicate: Rep 2
flow markers: CD69+ mixed with CD69-CD8+CD4+ and CD69- CD8- CD4-
cell type: 8:1:1 mixture of sorted CD69+, CD69- DP, and CD69- DN thymocytes
genotype: Zfp148 fl/fl; Zfp281 fl/fl; Cd4-Cre+
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Single Cell NextGem 5' V1.1 chemistry from 10X genomics
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
NextSeq 2000 |
| |
|
| Data processing |
The standard 10x Genomics cellranger (version 5.0.1) pipeline was used to extract Fastqs and 10X Genomics cellranger (version 5.0.1) pipeline was used to perform data processing.
Sequenced reads were aligned to the 10x Genomics provided mouse reference sequence (refdata-gex-mm10-2020-A).
Assembly: mm10
Supplementary files format and content: h5 file; Feature and barcode matrix
|
| |
|
| Submission date |
Jun 21, 2022 |
| Last update date |
Nov 10, 2023 |
| Contact name |
Remy Bosselut |
| E-mail(s) |
bosselur@helix.nih.gov
|
| Phone |
240-760-6866
|
| Organization name |
National Cancer Institute, National Institute of Health
|
| Department |
Laboratory of Immune Cell Biology
|
| Lab |
Bosselut
|
| Street address |
9000 Rockville Pike, Building 37
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platform ID |
GPL30172 |
| Series (2) |
| GSE206521 |
The transcription factor Zfp281 serves redundantly with Zfp148 to support CD4+ T cell development and functions [scRNA-seq] |
| GSE207572 |
Zfp281 and Zfp148 control CD4+ T cell thymic development and Th2 functions |
|
| Relations |
| BioSample |
SAMN29220529 |
| SRA |
SRX15805593 |
