|
| Status |
Public on Jan 01, 2024 |
| Title |
KO, Spleen, 2- 3 |
| Sample type |
SRA |
| |
|
| Source name |
Spleen
|
| Organism |
Mus musculus |
| Characteristics |
cell type: T cell
tissue: Spleen
strain: C57BL/6
age: 9w
treatment: o-transfer cKO CD8+ T cells
infection: Lymphocytic choriomeningitis virus Clone 13 strain
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Lung and spleen of co-transfer WT and cKO CD8+ T cells were purified by FACS sorting.
Single-cell cDNA libraries were prepared using the MARS-Seq method, as described (Jaitin et al., 2014; Keren-Shaul et al., 2019)
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic single cell |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Description |
Mars-seq
cell_metadata.csv
|
| Data processing |
ScRNA-seq libraries (pooled at equimolar concentrations) were sequenced on an Illumina NextSeq 500.MARS-seq reads were processed using the MARS-seq2.0 pipeline (Keren-Shaul et al., 2019).
Assembly: mm10
Supplementary files format and content: Tab-separated values files and matrix files
|
| |
|
| Submission date |
Jun 14, 2022 |
| Last update date |
Jan 01, 2024 |
| Contact name |
ChangChun Xiao |
| E-mail(s) |
cxiao@scripps.edu
|
| Organization name |
The Scripps Research Institute
|
| Department |
Department of Immunology and Microbiology
|
| Street address |
North Torrey Pines Road
|
| City |
La Jolla |
| ZIP/Postal code |
CA 92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL19057 |
| Series (1) |
| GSE206067 |
Themis directs T cell exhaustion by promoting PD-1 signaling and key transcription factors TCF-1 and TOX expression |
|
| Relations |
| BioSample |
SAMN29038953 |
| SRA |
SRX15697611 |
