|
| Status |
Public on May 01, 2011 |
| Title |
Leaves_DEX_24h_3 |
| Sample type |
RNA |
| |
|
| Source name |
Leaves of transgenic Poplar carrying 35S:AtVND7-VP16-GR treated with 10µM DEX for 24 hours.
|
| Organism |
Populus tremula x Populus tremuloides |
| Characteristics |
tissue: Leaves
genotype: transgenic Poplar carrying 35S:AtVND7-VP16-GR
treatment: DEX
|
| Treatment protocol |
Leaves were cut into pieces with 1 cm width and then soaked in the water containing 0.033% EtOH as ccontrol, or 10 µ DEX solution for 24 hours. EtOH was a solvent for DEX stock solution.
|
| Growth protocol |
Poplar transgenic plants were grown aseptically on medium containing Murashige and Skoog (MS) salt mixture under SD (8 hours light/16 hours dark) conditions at 23°C.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed by Plant RNA Isolation Reagent (Invitrogen), and then total RNA was purified by RNeasy Mini Kit (QIAGEN), according to the manufacturer's instructions.
|
| Label |
Cy3
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 ug total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 15 ug of cRNA were hybridized for 16 hr at 45C on GeneChip. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000.
|
| Description |
Leaves were cut into pieces with 1 cm width and then soaked in 10µM DEX solution for 24 hours. EtOH was a solvent for DEX stock solution. Replicate 3 of 3.
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Nov 12, 2010 |
| Last update date |
May 01, 2011 |
| Contact name |
Misato Ohtani |
| E-mail(s) |
misato@bs.naist.jp
|
| Phone |
+81-743-72-5462
|
| Organization name |
NAIST
|
| Department |
Graduate School of Biological Sciences
|
| Street address |
8916-5 Takayama-cho
|
| City |
Ikoma |
| ZIP/Postal code |
630-0192 |
| Country |
Japan |
| |
|
| Platform ID |
GPL4359 |
| Series (1) |
| GSE25304 |
Isolation of poplar genes involved in xylem vessel differentiation |
|
